Quan S, Yamano S, Nakasaka H, Hinokio K, Nagagawa K, Aono T
Department of Obstetrics and Gynecology, School of Medicine, University of Tokushima, Japan.
J Assist Reprod Genet. 2000 Aug;17(7):388-92. doi: 10.1023/a:1009449926015.
The aim was to assess the fertilizing capacity of spermatozoa cool-preserved in electrolyte-free (EF) solution.
Mouse spermatozoa were cool-preserved in EF solution and the acrosomal status of the spermatozoa was compared before and after preservation using chlortetracycline stain. Mouse oocytes were inseminated by spermatozoa cool-preserved in EF solution for 2, 4, or 7 days and fertilization and blastocyst rates were evaluated.
Acrosomal status of spermatozoa cool-preserved in EF solution was not different from spermatozoa before preservation, but the capacitated and acrosome-reacted spermatozoa significantly increased after reinitiation. Cool-preservation in EF solution for up to 4 days did not affect fertilization rate. Blastocyst rate of embryos derived from spermatozoa cool-preserved for 4 or 7 days in EF solution was significantly lower than that of embryos derived from fresh spermatozoa.
Mouse spermatozoa cool-preserved in EF solution possesses as much fertilizing capacity as fresh spermatozoa. However, prolonged preservation affects the embryonic development.
评估在无电解质（EF）溶液中冷藏保存的精子的受精能力。
将小鼠精子保存在EF溶液中，使用金霉素染色比较保存前后精子的顶体状态。用在EF溶液中冷藏保存2、4或7天的精子对小鼠卵母细胞进行授精，并评估受精率和囊胚率。
保存在EF溶液中的精子顶体状态与保存前的精子无差异，但重新启动后获能和顶体反应的精子显著增加。在EF溶液中冷藏保存长达4天不影响受精率。在EF溶液中冷藏保存4或7天的精子所衍生胚胎的囊胚率显著低于新鲜精子所衍生胚胎的囊胚率。
保存在EF溶液中的小鼠精子具有与新鲜精子一样多的受精能力。然而，延长保存时间会影响胚胎发育。
