Skibińska Maria, Kosmala Arkadiusz, Humphreys Michael W, Zwierzykowski Zbigniew
Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland.
Cell Mol Biol Lett. 2002;7(2A):493-8.
At present, breeding programmes aimed at combining advantageous traits within the Lolium-Festuca complex, are mainly focused on introgression procedures. One principal objective, is the transfer of genes conferring resistance to abiotic stresses from Festuca species (F. pratensis, F. arundinacea and F. glaucescens) into Lolium multiflorum and L. perenne germplasm. In our experiments, two different hybrids: triploid - L. multiflorum (4x) x F. pratensis (2x) and pentaploid - F. arundinacea (6x) x L. multiflorum (4x) were backcrossed twice onto L. multiflorum cultivars, and numerous BC2 progeny generated. BC2 plants from both combinations were tested in field and/or simulated conditions for winter hardiness and drought resistance. GISH (genomic in situ hybridisation) analyses were then performed on the most winter hardy and drought resistant plants to locate putative genes for stress resistance. Using resistant L. multiflorum genotypes with a single Festuca chromatin segment, it was possible to allocate AFLP (amplified fragment length polymorphism) markers specific to that segment. Markers associated with genes conferring stress resistance facilitate marker-assisted selection programmes to obtain new, more persistent grass cultivars. Preliminary results of GISH analysis, to identify Festuca chromosome segments in L. multiflorum introgression lines and to find segment-specific AFLP markers, are presented
目前，旨在将黑麦草 - 羊茅复合体中的有利性状进行组合的育种计划，主要集中在渐渗程序上。一个主要目标是将赋予对非生物胁迫抗性的基因从羊茅属物种（草地羊茅、高羊茅和 glaucescens 羊茅）转移到多花黑麦草和多年生黑麦草种质中。在我们的实验中，两种不同的杂种：三倍体 - 多花黑麦草（4x）×草地羊茅（2x）和五倍体 - 高羊茅（6x）×多花黑麦草（4x）与多花黑麦草品种回交了两次，并产生了大量的 BC2 后代。对来自这两种组合的 BC2 植株在田间和/或模拟条件下进行了抗寒性和抗旱性测试。然后对最抗寒和抗旱的植株进行基因组原位杂交（GISH）分析，以定位假定的抗逆基因。利用具有单个羊茅染色质片段的多花黑麦草抗性基因型，有可能分配特定于该片段的扩增片段长度多态性（AFLP）标记。与赋予抗逆性的基因相关的标记有助于标记辅助选择计划，以获得新的、更持久的草品种。本文展示了 GISH 分析的初步结果，以鉴定多花黑麦草渐渗系中的羊茅染色体片段并找到片段特异性 AFLP 标记。
