Szczepaniak Magdalena, Cieślak Elzbieta, Bednarek Piotr Tomasz
W. Szafer Institute of Botany, Polish Academy of Sciences, Department of Plant Systematics, Lubicz 42, 31-512 Kraków, Poland.
Cell Mol Biol Lett. 2002;7(2A):547-58.
Combined morphological and molecular techniques were used to characterize variation in Elymus repens. We studied the morphological variability of E. repens in relation to the degree of its genetic differentiation, in order to unravel the causes of conspicuous intraspecific morphological variation. Four populations of E. repens from different habitats were analyzed for 35 morphological characters, and their genetic differentiation was assessed by Amplified Fragment Length Polymorphism (AFLP). Four pairs of selective primers were used to detect a total of 279 AFLP bands, of which 104 (37.28%) were polymorphic between populations. Cluster analysis based on AFLP fingerprint data showed that individuals were arranged in population-specific groups. The analyses of variance (ANOVA and AMOVA) indicated significant morphological and genetic differentiation among populations (P<0.01). This study revealed low levels of AFLP variation, which suggests that conspicuous morphological variation of E. repens is caused by plasticity. E. repens is an evolutionarily young species, of hybrid origin, in which microevolutionary processes continue. This study showed that common analysis of genetic diversity and morphology is a powerful tool in low-level taxonomy.
结合形态学和分子技术对匍匐披碱草的变异特征进行了研究。我们研究了匍匐披碱草的形态变异性及其遗传分化程度之间的关系，以揭示种内显著形态变异的原因。对来自不同生境的4个匍匐披碱草种群的35个形态特征进行了分析，并通过扩增片段长度多态性（AFLP）评估了它们的遗传分化。使用4对选择性引物共检测到279条AFLP条带，其中104条（37.28%）在种群间具有多态性。基于AFLP指纹数据的聚类分析表明，个体按种群特异性分组排列。方差分析（ANOVA和AMOVA）表明种群间存在显著的形态和遗传分化（P<0.01）。本研究揭示了AFLP变异水平较低，这表明匍匐披碱草显著的形态变异是由可塑性引起的。匍匐披碱草是一个起源于杂交的进化上较年轻的物种，其中微观进化过程仍在继续。本研究表明，遗传多样性和形态的综合分析是低水平分类学中的一个有力工具。
