Zhang Lei, Huang Bei-Bei, Kai Guo-Yin, Guo Mei-Li
School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Yao Xue Xue Bao. 2006 Jan;41(1):91-6.
To investigate the intraspecific variation of Carthamus tinctorius L. (safflower) and establish foundation for further breeding of safflower germplasm resource and screening the quality correlation genes.
Amplified fragment length polymorphism (AFLP) was carried out to analyze genetic variation of 28 safflower populations collected in China. Unweighed pair-group method of with arithmetical averages (UPGMA) cluster analysis was used to construct a dendrogram and to estimate the genetic distances among the populations.
All populations could be uniquely distinguished using 12 selected primer combinations. Similarity coefficients ranged from 0.48 to 0.96 among the populations. Dendrogram revealed distinct segregation of all the cultivars into three main groups and one midst group.
Limited genetic diversity exists within the tested 28 collections at intra specific level and AFLP-based phyiogeny was not absolutely consistent with that based on morphological characters may be due to the interaction effect between genotype and environment.
研究红花（Carthamus tinctorius L.）种内变异，为红花种质资源进一步育种及筛选品质相关基因奠定基础。
采用扩增片段长度多态性（AFLP）分析中国收集的28个红花种群的遗传变异。运用非加权算术平均法（UPGMA）聚类分析构建树状图，并估计种群间的遗传距离。
使用12对选定的引物组合可唯一区分所有种群。种群间的相似系数范围为0.48至0.96。树状图显示所有品种明显分为三个主要组和一个中间组。
在测试的28个收集品中，种内水平存在有限的遗传多样性，基于AFLP的系统发育与基于形态特征的系统发育不完全一致，这可能是由于基因型与环境之间的相互作用效应。
