Szczepaniak M, Bieniek W, Boroń P, Szklarczyk M, Mizianty M
Department of Vascular Plant Systematics, Polish Academy of Sciences, W. Szafer Institute of Botany, Kraków, Poland.
Plant Biol (Stuttg). 2009 Sep;11(5):766-73. doi: 10.1111/j.1438-8677.2008.00171.x.
To determine the relative importance of clonal growth and sexual reproduction, the Randomly Amplified Polymorphic DNA (RAPD) method was used to study genetic diversity and clonal structure of six populations of Elymus repens and four populations of Elymus hispidus from Poland. These outbreeding species are virtually self-sterile and form widely spreading and long-lived rhizomes. Using 12 primers, a total of 150 unambiguous RAPD fragments were amplified and scored. Results of AMOVA showed no significant genetic distinction between morphologically distinguished varieties of E. repens and E. hispidus. E. repens had slightly higher intra-specific genetic polymorphism than E. hispidus; the percentage of polymorphic bands per population ranged from 38 to 49 and from 19 to 38 respectively. Clonal diversity measured using the Simpson diversity index (D) indicated different contributions of clonal reproduction in particular populations of E. repens (D: 0.20-0.72). Populations of E. hispidus were dominated by one or a few clones, which were generally restricted to a single population (D: 0.00-0.22). RAPD revealed that most genetic diversity resided within populations of the two studied species, suggesting that, despite their clonal character, propagation by seeds contributes considerably to reproduction of E. repens and E. hispidus.
为了确定克隆生长和有性繁殖的相对重要性，采用随机扩增多态性DNA（RAPD）方法，对来自波兰的6个偃麦草种群和4个硬直偃麦草种群的遗传多样性和克隆结构进行了研究。这些异交物种几乎是自交不育的，并且形成广泛蔓延且长寿的根茎。使用12个引物，共扩增并记录了150个明确的RAPD片段。方差分析结果表明，形态上有区别的偃麦草和硬直偃麦草变种之间没有显著的遗传差异。偃麦草的种内遗传多态性略高于硬直偃麦草；每个种群的多态性条带百分比分别为38%至49%和19%至38%。使用辛普森多样性指数（D）测量的克隆多样性表明，克隆繁殖在偃麦草特定种群中的贡献不同（D：0.20 - 0.72）。硬直偃麦草种群由一个或几个克隆主导，这些克隆通常局限于单个种群（D：0.00 - 0.22）。RAPD分析表明，两个研究物种的大多数遗传多样性存在于种群内部，这表明，尽管它们具有克隆特性，但种子繁殖对偃麦草和硬直偃麦草的繁殖有相当大的贡献。
