Suliman-Pollatschek Saskia, Kashkush Khalil, Shats Hadas, Hillel Jossi, Lavi Uri
MBC, 5 Oppenheimer St. Science Park Rehovot P.O.B 4018 Nes- Ziona 70400, Israel.
Cell Mol Biol Lett. 2002;7(2A):583-97.
Amplified Fragment Length Polymorphism (AFLP), Simple Sequence Repeat (SSR) and Single Nucleotide Polymorphism (SNP), were applied to the tomato genome for assessment of polymorphism and for mapping. The polymorphism of AFLP was studied in twenty-one commercial tomato (L. esculentum) varieties. Four AFLP primer combinations produced 298 clear bands; an average of 75 bands per combination. SSR markers were generated from two sources: (1) size-selected genomic libraries screened with (AT)n, (CT)n, (GT)n, (ATT)n and (CTT)n probes. (2) GeneBank database. Primers were designed for 114 loci and used for genotyping 13 tomato varieties and three Lycopersicon species. Eighteen markers were used to evaluate the polymorphism among the commercial cultivars and were found to be a useful tool for cultivar identification. In-silico comparison of DNA sequences (ESTs and genes) of L. pennellii and L. esculentum, yielded 312 SNPs. Ten L. pennelli genomic fragments were sequenced and the comparison with L. esculentum yielded 22 SNPs. Another 19 SNPs were discovered by sequencing and comparing L. pennellii genomic DNA to L. esculentum DNA fragments containing SSRs. The average SNP frequency was found to be one in a few tens of base pairs. A total of 52 microsatellites, 159 polymorphic AFLP markers and six SNPs were mapped using the Introgression Lines generated by [1]. Map location and markers' distribution are presented.
扩增片段长度多态性（AFLP）、简单序列重复（SSR）和单核苷酸多态性（SNP）被应用于番茄基因组，以评估多态性并进行图谱绘制。在21个商业番茄（L. esculentum）品种中研究了AFLP的多态性。四种AFLP引物组合产生了298条清晰的条带；平均每个组合75条带。SSR标记来自两个来源：（1）用（AT）n、（CT）n、（GT）n、（ATT）n和（CTT）n探针筛选的大小选择基因组文库。（2）基因库数据库。为114个位点设计了引物，并用于对13个番茄品种和三个番茄属物种进行基因分型。18个标记用于评估商业品种之间的多态性，被发现是品种鉴定的有用工具。对潘那利番茄（L. pennellii）和栽培番茄（L. esculentum）的DNA序列（EST和基因）进行电子比较，产生了312个SNP。对10个潘那利番茄基因组片段进行了测序，并与栽培番茄进行比较，产生了22个SNP。通过对潘那利番茄基因组DNA与含有SSR的栽培番茄DNA片段进行测序和比较，又发现了19个SNP。发现平均SNP频率为几十对碱基中有一个。使用由[1]产生的渐渗系，共定位了52个微卫星、159个多态性AFLP标记和6个SNP。给出了图谱位置和标记分布。
