荧光胺在蛋白质 - DNA 相互作用研究中的应用。
Application of fluorescamine to the study of protein-DNA interactions.
作者信息
Bode J
出版信息
Nucleic Acids Res. 1975 Oct;2(10):1951-65. doi: 10.1093/nar/2.10.1951.
Abstract
The reactivity of alpha-amino groups of basic proteins towards fluorescamine is essentially abolished if salt linkages with DNA phosphate groups are formed. This observation prompted the elaboration of a very general assay which allows the determination of binding parameters for the interaction of proteins with DNA and chromatin. Protamines, labeled with fluorescamine prior to their binding by DNA appear to be useful probes to monitor the formation and nature of DNA-protein complexes.
摘要
如果碱性蛋白质的α-氨基与DNA磷酸基团形成盐键，其对荧光胺的反应活性基本消失。这一观察结果促使人们精心设计了一种非常通用的测定方法，该方法可用于测定蛋白质与DNA及染色质相互作用的结合参数。在被DNA结合之前用荧光胺标记的鱼精蛋白似乎是监测DNA-蛋白质复合物形成及其性质的有用探针。
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2
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Biochemistry. 1968 Jun;7(6):2192-201. doi: 10.1021/bi00846a023.
3
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4
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