Bode J, Willmitzer L, Opatz K
Eur J Biochem. 1977 Jan;72(2):393-403. doi: 10.1111/j.1432-1033.1977.tb11264.x.
Specific contacts between the crossinteracting histones effectively prevent their protamine-mediated displacement from nucleoproteins revealing a complex competition pattern not expected from their relative affinities to DNA. Histone H1 is the only species freed under a variety of conditions if native chromatin is used; the results found with chromatins obtained by conventional methods reflect the extent to which histone redistribution has occurred. It is concluded that the events occurring during spermiogenesis must be apt to disrupt histone interactions found in the nucleosomes. The possible role of a protamine phosphorylation/dephosphorylation mechanism is discussed on the basis of comparative experiments with protamines phosphorylated to different extents.
相互作用的组蛋白之间的特定接触有效地阻止了它们被鱼精蛋白从核蛋白中置换出来，揭示了一种复杂的竞争模式，这是根据它们与DNA的相对亲和力所无法预期到的。如果使用天然染色质，组蛋白H1是在各种条件下唯一能被释放出来的种类；用传统方法获得的染色质所得到的结果反映了组蛋白重新分布的程度。得出的结论是，精子发生过程中发生的事件必定易于破坏核小体中发现的组蛋白相互作用。基于对不同程度磷酸化的鱼精蛋白进行的比较实验，讨论了鱼精蛋白磷酸化/去磷酸化机制的可能作用。
