Yaïci E-D, Rampin O, Calas A, Jestin A, McKenna K E, Leclerc P, Benoit G, Giuliano F
Groupe de Recherche en Urologie, UPRES EA 1602, Faculté de Médecine Paris-Sud, 63 rue Gabriel Péri, 94270 Le Kremlin-Bicêtre, France.
Neuroscience. 2002;114(4):945-60. doi: 10.1016/s0306-4522(02)00367-6.
The thoracolumbar and lumbosacral spinal cord contain respectively sympathetic and parasympathetic preganglionic neurons that supply the organs of the pelvis including the penis. These neurons are influenced by supraspinal information and receive aminergic projections from the brainstem. The presence of the alpha(1)- and alpha(2)-adrenoceptor subtypes has been demonstrated in the rat spinal cord. In this species, we looked for the presence of alpha(2a)- and alpha(2c)-adrenoceptor subtypes in the sympathetic and parasympathetic preganglionic neurons controlling erection. In adult male rats, transsynaptic axonal transport of pseudorabies virus injected into the penis was combined with immunohistochemistry against alpha(2a)- and alpha(2c)-adrenoceptor subtypes. At 4 days survival time, neurons infected with the pseudorabies virus were solely found in the intermediolateral cell column and dorsal gray commissure of segment T12-L2 and in the intermediolateral cell column of segment L6-S1. Neurons and fibers immunoreactive for alpha(2a)- and alpha(2c)-adrenoceptor subtypes were mainly present in the intermediolateral cell column, the dorsal gray commissure and the ventral horn of the T12-L2 and L5-S1 spinal cord, the dorsal horn displayed only immunoreactive fibers. Pseudorabies virus-infected neurons in the autonomic nuclei were both immunoreactive for alpha(2a)- and alpha(2c)-adrenoceptor subtypes and closely apposed by alpha(2a)- and alpha(2c)-immunoreactive fibers. The results suggest an intraspinal modulation of the noradrenergic and adrenergic control of the autonomic outflow to the penis by pre- and postsynaptic alpha(2) adrenoceptors.
胸腰段和腰骶段脊髓分别包含支配包括阴茎在内的盆腔器官的交感和副交感神经节前神经元。这些神经元受脊髓上信息的影响，并接受来自脑干的胺能投射。α(1)-和α(2)-肾上腺素能受体亚型已在大鼠脊髓中得到证实。在这个物种中，我们寻找在控制勃起的交感和副交感神经节前神经元中α(2a)-和α(2c)-肾上腺素能受体亚型的存在情况。在成年雄性大鼠中，将注射到阴茎的伪狂犬病病毒的跨突触轴突运输与针对α(2a)-和α(2c)-肾上腺素能受体亚型的免疫组织化学相结合。在存活4天时，仅在T12-L2节段的中间外侧细胞柱和背侧灰质连合以及L6-S1节段的中间外侧细胞柱中发现感染伪狂犬病病毒的神经元。对α(2a)-和α(2c)-肾上腺素能受体亚型具有免疫反应性的神经元和纤维主要存在于T12-L2和L5-S1脊髓的中间外侧细胞柱、背侧灰质连合和腹角，背角仅显示免疫反应性纤维。自主神经核中感染伪狂犬病病毒的神经元对α(2a)-和α(2c)-肾上腺素能受体亚型均具有免疫反应性，并且紧密邻接α(2a)-和α(2c)-免疫反应性纤维。结果表明，突触前和突触后α(2)肾上腺素能受体对阴茎自主神经传出的去甲肾上腺素能和肾上腺素能控制进行脊髓内调节。
