Bancila M, Vergé D, Rampin O, Backstrom J R, Sanders-Bush E, McKenna K E, Marson L, Calas A, Giuliano F
Département de Neurobiologie des Signaux Intercellulaires, Institut des Neurosciences, CNRS UMR 7624, Université Pierre et Marie Curie, Paris, France.
Neuroscience. 1999;92(4):1523-37. doi: 10.1016/s0306-4522(99)00082-2.
The localization of 5-hydroxytryptamine2C receptors in the lumbosacral spinal cord of the rat was investigated using selective antibodies raised against the carboxyl-terminal part of the rat receptor. The distribution of immunoperoxidase labelling at the light microscope level revealed numerous labelled neurons in the gray matter, with a higher intensity in the sacral parasympathetic nucleus, the dorsal gray commissure and particularly the motoneurons of the ventral horn. Confocal microscope analysis showed that immunostaining was mainly intracellular (motoneurons), but could also be associated with the membrane of cell bodies and dendrites. Actually, electron microscope immunogold experiments demonstrated an exclusive staining of the cis-Golgi apparatus. Following pseudo-rabies virus transsynaptic retrograde labelling from the corpus cavernosum, labelled neurons were found in the sacral parasympathetic nucleus and the dorsal gray commissure of the L6-S1 segments. All virus-labelled neurons exhibited 5-hydroxytryptamine2C receptor immunoreactivity. These results indicate that all parasympathetic preganglionic neurons and their related interneurons which contribute to the innervation of cavernosal tissue bear 5-hydroxytryptamine2C receptors. In the sacral parasympathetic nucleus, most neurons which were retrogradely-labelled from the pelvic ganglion with Fast Blue also showed 5-hydroxytryptamine2C receptor immunoreactivity. In the ventral horn, motoneurons retrogradely labelled from the ischiocavernosus muscle and the bulbospongiosus muscle, both of which are involved in erection and ejaculation, were also 5-hydroxytryptamine2C receptor-immunopositive. The supraspinal serotoninergic control of erection at the lumbosacral level therefore appears to be strongly associated with the activation of 5-hydroxytryptamine2C receptors, consistent with the proerectile properties of 5-hydroxytryptamine2C agonists.
利用针对大鼠5-羟色胺2C受体羧基末端部分产生的特异性抗体，研究了该受体在大鼠腰骶脊髓中的定位。光镜水平的免疫过氧化物酶标记分布显示，灰质中有大量标记神经元，在骶副交感核、背侧灰质连合，尤其是腹角运动神经元中强度更高。共聚焦显微镜分析表明，免疫染色主要在细胞内（运动神经元），但也可能与细胞体和树突的膜相关。实际上，电子显微镜免疫金实验证明，顺式高尔基体有特异性染色。从海绵体进行伪狂犬病病毒跨突触逆行标记后，在L6-S1节段的骶副交感核和背侧灰质连合中发现了标记神经元。所有病毒标记的神经元均表现出5-羟色胺2C受体免疫反应性。这些结果表明，所有参与海绵体组织神经支配的副交感神经节前神经元及其相关中间神经元都带有5-羟色胺2C受体。在骶副交感核中，大多数用快蓝从盆腔神经节逆行标记的神经元也表现出5-羟色胺2C受体免疫反应性。在腹角，从坐骨海绵体肌和球海绵体肌逆行标记的运动神经元，这两块肌肉都参与勃起和射精，也为5-羟色胺2C受体免疫阳性。因此，腰骶水平对勃起的脊髓上5-羟色胺能控制似乎与5-羟色胺2C受体的激活密切相关，这与5-羟色胺2C激动剂的促勃起特性一致。
