Miller S, Walker S W, Arthur J R, Lewin M H, Pickard K, Nicol F, Howie A F, Beckett G J
Clinical Biochemistry, University of Edinburgh, Royal Infirmary, Lauriston Place, Edinburgh, UK.
Biochim Biophys Acta. 2002 Oct 9;1588(1):85-93. doi: 10.1016/s0925-4439(02)00143-6.
Selenium (Se) can protect endothelial cells (EC) from oxidative damage by altering the expression of selenoproteins with antioxidant function such as cytoplasmic glutathione peroxidase (cyGPX), phospholipid hydroperoxide glutathione peroxidase (PHGPX) and thioredoxin reductase (TR). If the role of Se on EC function is to be studied, it is essential that a model system be chosen which reflects selenoprotein expression in human EC derived from vessels prone to developing atheroma. We have used [75Se]-selenite labelling and selenoenzyme measurements to compare the selenoproteins expressed by cultures of EC isolated from different human vasculature with EC bovine and porcine aorta. Only small differences were observed in selenoprotein expression and activity in EC originating from human coronary artery, human umbilical vein (HUVEC), human umbilical artery and the human EC line EAhy926. The selenoprotein profile in HUVEC was consistent over eight passages and HUVEC isolated from four cords also showed little variability. In contrast, EC isolated from pig and bovine aorta showed marked differences in selenoprotein expression when compared to human cells. This study firmly establishes the suitability and consistency of using HUVEC (and possibly the human cell line EAhy926) as a model to study the effects of Se on EC function in relation to atheroma development in the coronary artery. Bovine or porcine EC appear to be an inappropriate model.
硒（Se）可通过改变具有抗氧化功能的硒蛋白（如胞质谷胱甘肽过氧化物酶（cyGPX）、磷脂氢谷胱甘肽过氧化物酶（PHGPX）和硫氧还蛋白还原酶（TR））的表达来保护内皮细胞（EC）免受氧化损伤。若要研究硒对内皮细胞功能的作用，必须选择一个能反映源自易发生动脉粥样硬化血管的人内皮细胞中硒蛋白表达的模型系统。我们已使用[75Se]亚硒酸盐标记和硒酶测量方法，比较从不同人体脉管系统分离的内皮细胞培养物与牛和猪主动脉内皮细胞所表达的硒蛋白。在源自人冠状动脉、人脐静脉（HUVEC）、人脐动脉和人内皮细胞系EAhy926的内皮细胞中，仅观察到硒蛋白表达和活性存在微小差异。HUVEC中的硒蛋白谱在八代培养过程中保持一致，从四条脐带分离的HUVEC也显示出很小的变异性。相比之下，与人类细胞相比，从猪和牛主动脉分离的内皮细胞在硒蛋白表达上存在显著差异。这项研究明确证实，使用HUVEC（可能还有人细胞系EAhy926）作为模型来研究硒对内皮细胞功能的影响以及与冠状动脉粥样硬化发展的关系是合适且一致的。牛或猪内皮细胞似乎是不合适的模型。
