Maiorino M, Chu F F, Ursini F, Davies K J, Doroshow J H, Esworthy R S
Dipartimento di Chimica Biologica, Università di Padova, Italy.
J Biol Chem. 1991 Apr 25;266(12):7728-32.
Human tumor cell lines cultured in 75Se-containing media demonstrate four major 75Se-labeled cellular proteins (57, 22, 18, and 12 kDa) on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. Among these selenoproteins, an enzymatic activity is known only for the 22-kDa protein, since this protein has been identified as the monomer of glutathione peroxidase. However, all tested cell lines also contained a peroxidase activity with phospholipid hydroperoxides that is completely accounted for by the other selenoenzyme, phospholipid hydroperoxide glutathione peroxidase (PHGPX) (Ursini, F., Maiorino, M., and Gregolin, C. (1985) Biochim. Biophys. Acta 839, 62-70). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography of 75Se-labeled proteins separated by gel permeation chromatography supported the identification of PHGPX as the monomeric protein matching the 18 kDa band. This paper is the first report on the identification of PHGPX in human cells.
在含75Se的培养基中培养的人肿瘤细胞系，经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和放射自显影显示出四种主要的75Se标记的细胞蛋白（57、22、18和12 kDa）。在这些硒蛋白中，仅22 kDa蛋白具有酶活性，因为该蛋白已被鉴定为谷胱甘肽过氧化物酶的单体。然而，所有测试的细胞系还含有一种对磷脂氢过氧化物的过氧化物酶活性，这完全由另一种硒酶——磷脂氢过氧化物谷胱甘肽过氧化物酶（PHGPX）所承担（乌尔西尼，F.，马约里诺，M.，和格雷戈林，C.（1985年）《生物化学与生物物理学学报》839，62 - 70）。通过凝胶渗透色谱分离的75Se标记蛋白的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和放射自显影支持将PHGPX鉴定为与18 kDa条带匹配的单体蛋白。本文是关于在人细胞中鉴定PHGPX的首次报道。
