Trutic Natasa, Magic Zvonko, Urosevic Nadezda, Krtolica Koviljka
Department of Medical Chemistry, Faculty of Medicine, University of Nis, Nis, Yugoslavia.
Comp Biochem Physiol C Toxicol Pharmacol. 2002 Nov;133(3):461-70. doi: 10.1016/s1532-0456(02)00174-6.
We studied the effect of total body X-irradiation and partial hepatectomy on the acute phase protein gene expression in rat liver. Male rats of AO strain were irradiated with high X-ray doses, without any visible tissue damage. In contrast, partial hepatectomy consisted of surgical removal of 40% liver tissue. The changes in liver mRNA concentrations for positive acute-phase reactants including cysteine protease inhibitor, alpha(1)-acid glycoprotein, fibrinogen and haptoglobin, and albumin as a negative reactant were monitored by Northern blot and slot-blot hybridizations using corresponding [32P]dCTP labeled cDNA probes. While in the first 24 h after the partial hepatectomy, liver mRNA levels for the positive acute-phase reactants increased, briefly followed by an immediate decrease, the duration and timing of the acute-phase responses to the whole body X-irradiation were slightly different and lasted for as long as 72 h. Although both treatments induced the mRNA expression of acute-phase reactants in rat liver, the observed variations in the duration and intensity of the changes in mRNA levels for the acute-phase proteins in these two types of tissue damage suggest the involvement of specific mechanisms in a fine tuning of the non-specific acute-phase responses to meet the unique requirements of the particular injury.
我们研究了全身X射线照射和部分肝切除对大鼠肝脏急性期蛋白基因表达的影响。AO品系的雄性大鼠接受高剂量X射线照射，未出现任何可见的组织损伤。相比之下，部分肝切除是通过手术切除40%的肝组织。使用相应的[32P]dCTP标记的cDNA探针，通过Northern印迹和狭缝印迹杂交监测肝脏中包括半胱氨酸蛋白酶抑制剂、α(1)-酸性糖蛋白、纤维蛋白原和触珠蛋白等阳性急性期反应物以及作为阴性反应物的白蛋白的mRNA浓度变化。在部分肝切除后的最初24小时内，阳性急性期反应物的肝脏mRNA水平升高，随后短暂下降，而全身X射线照射引起的急性期反应的持续时间和时间略有不同，持续长达72小时。虽然两种处理均诱导了大鼠肝脏中急性期反应物的mRNA表达，但在这两种类型的组织损伤中，急性期蛋白mRNA水平变化的持续时间和强度的观察差异表明，特定机制参与了非特异性急性期反应的精细调节，以满足特定损伤的独特需求。
