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迟缓爱德华氏菌胞外蛋白的比较蛋白质组学分析

Comparative proteomic analysis of extracellular proteins of Edwardsiella tarda.

作者信息

Tan Y P, Lin Q, Wang X H, Joshi S, Hew C L, Leung K Y

机构信息

Department of Biological Sciences, Faculty of Science. Tropical Marine Science Institute, the National University of Singapore, Singapore 117543.

出版信息

Infect Immun. 2002 Nov;70(11):6475-80. doi: 10.1128/IAI.70.11.6475-6480.2002.

DOI:10.1128/IAI.70.11.6475-6480.2002
PMID:12379732
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC130330/
Abstract

A comparison of extracellular proteins of virulent and avirulent Edwardsiella tarda strains revealed several major, virulent-strain-specific proteins. Proteomic analysis identified two of the proteins in the virulent strain PPD130/91 as flagellin and SseB, which are virulence factors in bacterial pathogens. PCR amplification and DNA sequencing confirmed the presence of the genes that encode these proteins. Our results clearly demonstrated the potency of the proteomic approach in identifying virulence factors.

摘要

对有毒和无毒迟缓爱德华氏菌菌株的细胞外蛋白质进行比较，发现了几种主要的、有毒菌株特有的蛋白质。蛋白质组学分析确定了有毒菌株PPD130/91中的两种蛋白质为鞭毛蛋白和SseB，它们是细菌病原体中的毒力因子。PCR扩增和DNA测序证实了编码这些蛋白质的基因的存在。我们的结果清楚地证明了蛋白质组学方法在识别毒力因子方面的有效性。

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J Bacteriol. 2001 Oct;183(20):6036-45. doi: 10.1128/JB.183.20.6036-6045.2001.
2
Opsonized virulent Edwardsiella tarda strains are able to adhere to and survive and replicate within fish phagocytes but fail to stimulate reactive oxygen intermediates.经调理素作用的强毒迟缓爱德华氏菌菌株能够黏附于鱼类吞噬细胞并在其中存活和复制，但无法刺激活性氧中间体的产生。
Infect Immun. 2001 Sep;69(9):5689-97. doi: 10.1128/IAI.69.9.5689-5697.2001.
3
Charting the proteomes of organisms with unsequenced genomes by MALDI-quadrupole time-of-flight mass spectrometry and BLAST homology searching.通过基质辅助激光解吸电离-四极杆飞行时间质谱法和BLAST同源性搜索对基因组未测序的生物体的蛋白质组进行图谱绘制。
Anal Chem. 2001 May 1;73(9):1917-26. doi: 10.1021/ac0013709.
4
Edwardsiella tarda mutants defective in siderophore production, motility, serum resistance and catalase activity.迟缓爱德华氏菌在铁载体产生、运动性、血清抗性和过氧化氢酶活性方面存在缺陷的突变体。
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5
Salmonella pathogenicity island 2.沙门氏菌致病岛2
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6
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7
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8
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9
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10
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