迟缓爱德华氏菌胞外蛋白的比较蛋白质组学分析
Comparative proteomic analysis of extracellular proteins of Edwardsiella tarda.
作者信息
Tan Y P, Lin Q, Wang X H, Joshi S, Hew C L, Leung K Y
机构信息
Department of Biological Sciences, Faculty of Science. Tropical Marine Science Institute, the National University of Singapore, Singapore 117543.
出版信息
Infect Immun. 2002 Nov;70(11):6475-80. doi: 10.1128/IAI.70.11.6475-6480.2002.
Abstract
A comparison of extracellular proteins of virulent and avirulent Edwardsiella tarda strains revealed several major, virulent-strain-specific proteins. Proteomic analysis identified two of the proteins in the virulent strain PPD130/91 as flagellin and SseB, which are virulence factors in bacterial pathogens. PCR amplification and DNA sequencing confirmed the presence of the genes that encode these proteins. Our results clearly demonstrated the potency of the proteomic approach in identifying virulence factors.
摘要
对有毒和无毒迟缓爱德华氏菌菌株的细胞外蛋白质进行比较，发现了几种主要的、有毒菌株特有的蛋白质。蛋白质组学分析确定了有毒菌株PPD130/91中的两种蛋白质为鞭毛蛋白和SseB，它们是细菌病原体中的毒力因子。PCR扩增和DNA测序证实了编码这些蛋白质的基因的存在。我们的结果清楚地证明了蛋白质组学方法在识别毒力因子方面的有效性。
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