Deiwick J, Hensel M
Lehrstuhl für Bakteriologie, Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie der Ludwig-Maximillians-Universität München, Munich, Germany.
Electrophoresis. 1999 Apr-May;20(4-5):813-7. doi: 10.1002/(SICI)1522-2683(19990101)20:4/5<813::AID-ELPS813>3.0.CO;2-Q.
Sensing and responding to environmental signals is a crucial element of bacterial pathogenicity. For a successful progression of infection, virulence gene expression is coordinated in response to habitat-specific environmental signals from the host organism. We are interested in identifying environmental cues affecting the expression of genes within Salmonella Pathogenicity Island 2 (SPI2), a virulence locus important for systemic infections by S. typhimurium. We describe our approach starting with the identification of new virulence genes, and analysis of the regulation of these genes by environmental signals leading to the proteome analysis in order to define the SPI2 regulon.
感知并响应环境信号是细菌致病性的关键要素。为使感染成功进展，毒力基因的表达会根据来自宿主生物体的特定栖息地环境信号进行协调。我们感兴趣的是确定影响鼠伤寒沙门氏菌2型致病岛（SPI2）内基因表达的环境线索，SPI2是鼠伤寒沙门氏菌全身感染的一个重要毒力位点。我们描述了我们的方法，首先是鉴定新的毒力基因，分析这些基因受环境信号的调控，进而进行蛋白质组分析以确定SPI2调控子。
