Nakamura Nobuki, Miyagi Eri, Murata Shin-ichi, Kawaoi Akira, Katoh Ryohei
Department of Pathology, Yamanashi Medical University, Yamanashi, Japan.
Mod Pathol. 2002 Oct;15(10):1058-67. doi: 10.1097/01.MP.0000028572.44247.CF.
The expression of thyroid transcription factor-1 in normal and neoplastic tissues and cell lines of the human lung was investigated using immunohistochemistry and in situ hybridization in conjunction with reverse transcription polymerase chain reaction. In normal lung tissues, immunoproducts of thyroid transcription factor-1 were observed in the nuclei of alveolar cells and bronchiolar cells. Interestingly, in distal bronchioles, immunohistochemistry and in situ hybridization revealed that thyroid transcription factor-1 was present not only in nonciliated cells (Clara cells) but also in ciliated cells and basal cells. In neoplastic tissues, thyroid transcription factor-1 was demonstrated in adenocarcinomas and small cell lung carcinomas with high frequency: 96% and 89% of cases, respectively. Thyroid transcription factor-1 was not detected in squamous cell carcinomas and large cell carcinomas. The strong immunoreactivity of thyroid transcription factor-1 or simultaneous expressions of thyroid transcription factor-1 and surfactant protein A tended to correlate with the differentiation phenotypes in adenocarcinomas; they were more frequently present in the well-differentiated type than were moderately and/or poorly differentiated types. By reverse transcription polymerase chain reaction, expression of thyroid transcription factor-1 messenger RNA was observed in squamous cell carcinomas in addition to in adenocarcinomas and small cell lung carcinomas, and this finding was confirmed in the cell lines from squamous cell carcinomas. Only one case of 99 adenocarcinomas that originated in various organs other than lung and thyroid immunohistochemically expressed thyroid transcription factor-1. Our results suggest that thyroid transcription factor-1 can play an important role for the maintenance and/or differentiation process in bronchiolar and alveolar cells. Thyroid transcription factor-1 expression associates with histologic types and/or differentiation of lung cancers and can be a valuable marker for the better understanding of their biological nature and pathological behavior.
采用免疫组织化学、原位杂交并结合逆转录聚合酶链反应，研究了甲状腺转录因子-1在人肺正常组织、肿瘤组织及细胞系中的表达情况。在正常肺组织中，甲状腺转录因子-1的免疫产物见于肺泡细胞和细支气管细胞的细胞核。有趣的是，在终末细支气管，免疫组织化学和原位杂交显示甲状腺转录因子-1不仅存在于无纤毛细胞（克拉拉细胞），也存在于纤毛细胞和基底细胞。在肿瘤组织中，甲状腺转录因子-1在腺癌和小细胞肺癌中高频率表达：分别为96%和89%的病例。在鳞状细胞癌和大细胞癌中未检测到甲状腺转录因子-1。甲状腺转录因子-1的强免疫反应性或甲状腺转录因子-1与表面活性蛋白A的同时表达倾向于与腺癌的分化表型相关；它们在高分化型中比中分化和/或低分化型更常见。通过逆转录聚合酶链反应，除腺癌和小细胞肺癌外，在鳞状细胞癌中也观察到甲状腺转录因子-1信使核糖核酸的表达，这一发现也在鳞状细胞癌细胞系中得到证实。99例非肺和甲状腺来源的腺癌中，只有1例免疫组织化学表达甲状腺转录因子-1。我们的结果表明，甲状腺转录因子-1在细支气管和肺泡细胞的维持和/或分化过程中可能起重要作用。甲状腺转录因子-1的表达与肺癌的组织学类型和/或分化相关，可能是更好理解其生物学特性和病理行为的有价值标志物。
