Llop-Tous Immaculada, Domínguez-Puigjaner Eva, Vendrell Miquel
Departmento de Fisiología y Biodiversidad Molecular, Instituto de Biología Molecular de Barcelona, CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain.
J Exp Bot. 2002 Nov;53(378):2283-5. doi: 10.1093/jxb/erf103.
A full-length cDNA clone (FaCDPK1) encoding a calcium-dependent protein kinase (CDPK) has been isolated from a strawberry fruit cDNA library. FaCDPK1 contains the basic features of CDPKs: a catalytic kinase domain linked to a regulatory calmodulin-like domain by a junction sequence that has been shown to act as an autoinhibitory pseudosubstrate. Although the calmodulin-like domain of CDPKs typically contains four EF-hand calcium-binding motifs, FaCDPK1 was predicted to contain only three EF-hand motifs. FaCDPK1 gene expression was observed in roots, stolons, meristems, flowers, and leaves. FaCDPK1 mRNA was not detected in young fruits, but accumulated as fruit turned to white, suggesting a role for this gene in the developing strawberry fruit. In ripe fruit the levels of transcript increased in response to low temperature.
从草莓果实cDNA文库中分离出了一个编码钙依赖性蛋白激酶（CDPK）的全长cDNA克隆（FaCDPK1）。FaCDPK1具有CDPK的基本特征：一个催化激酶结构域通过一个连接序列与一个调节性钙调蛋白样结构域相连，该连接序列已被证明可作为一种自抑制假底物。虽然CDPK的钙调蛋白样结构域通常包含四个EF手型钙结合基序，但预测FaCDPK1仅包含三个EF手型基序。在根、匍匐茎、分生组织、花和叶中观察到了FaCDPK1基因的表达。在幼果中未检测到FaCDPK1 mRNA，但随着果实变白其积累量增加，这表明该基因在草莓果实发育中起作用。在成熟果实中，转录本水平会因低温而升高。
