大鼠脑丙酮酸羧化酶的纯化及性质
Purification and properties of rat brain pyruvate carboxylase.
作者信息
Mahan D E, Mushahwar I K, Koeppe R E
出版信息
Biochem J. 1975 Jan;145(1):25-35. doi: 10.1042/bj1450025.
Abstract
Rat brain pyruvate carboxylase was purified 2000-fold and some of its properties and kinetic parameters were investigated. The use of (NH4)2SO4 gradient solubilization on a Celite column and precipitation with polyethylene glycol permitted purification to an estimated 20% purity. Except for a few subtle kinetic differences this enzyme is indistinguishable from rat liver pyruvate carboxylase.
摘要
大鼠脑丙酮酸羧化酶被纯化了2000倍，并对其一些性质和动力学参数进行了研究。在硅藻土柱上使用硫酸铵梯度溶解法以及用聚乙二醇沉淀法，可将其纯化至估计纯度为20%。除了一些细微的动力学差异外，这种酶与大鼠肝脏丙酮酸羧化酶没有区别。
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