Zeczycki Tonya N, Maurice Martin St, Attwood Paul V
Department of Biochemistry, University of Wisconsin, Madison, WI 53726, USA.
Open Enzym Inhib J. 2010;3:8-26. doi: 10.2174/1874940201003010008.
This review aims to discuss the varied types of inhibitors of biotin-dependent carboxylases, with an emphasis on the inhibitors of pyruvate carboxylase. Some of these inhibitors are physiologically relevant, in that they provide ways of regulating the cellular activities of the enzymes e.g. aspartate and prohibitin inhibition of pyruvate carboxylase. Most of the inhibitors that will be discussed have been used to probe various aspects of the structure and function of these enzymes. They target particular parts of the structure e.g. avidin - biotin, FTP - ATP binding site, oxamate - pyruvate binding site, phosphonoacetate - binding site of the putative carboxyphosphate intermediate.
本综述旨在讨论生物素依赖性羧化酶的多种抑制剂类型，重点是丙酮酸羧化酶的抑制剂。其中一些抑制剂具有生理相关性，因为它们提供了调节这些酶细胞活性的方式，例如天冬氨酸和抑制素对丙酮酸羧化酶的抑制作用。本文将讨论的大多数抑制剂已被用于探究这些酶结构和功能的各个方面。它们作用于结构的特定部位，例如抗生物素蛋白-生物素、FTP-ATP结合位点、草氨酸-丙酮酸结合位点、假定的羧基磷酸中间体的膦酰乙酸结合位点。
