Daniel P F, Wolf G
In Vitro. 1975 Nov-Dec;11(6):347-53. doi: 10.1007/BF02616370.
Hamster tracheas were cultured in serum-free CMRL 1066 medium buffered with either NaHCO3 alone or HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) plus NaHCO3 in an atmosphere of 95% O2 and 5% CO2. Afther 2 days in culture, tracheas maintained in HEPES plus NaHCO3-buffered medium showed an altered surface morphology. Histiological examination after 6 days of culture with HEPES plus NaHCO3 showed the presence of only scattered clumps of cilia. Incorporation of [14C]-glucosamine into intracellular glycoproteins was reduced by 75% and into secreted glycoproteins by 54% in cultures buffered with HEPES plus NaHCO3, compared to NaHCO3-buffered cultures. Incorporation of [H]fucose into intracellular glycoproteins was also reduced, although no effect was observed on secreted glycoproteins.
将仓鼠气管在无血清的CMRL 1066培养基中培养，该培养基用单独的NaHCO₃或HEPES（N - 2 - 羟乙基哌嗪 - N' - 2 - 乙磺酸）加NaHCO₃缓冲，培养环境为95% O₂和5% CO₂的气氛。培养2天后，维持在HEPES加NaHCO₃缓冲培养基中的气管表面形态发生改变。用HEPES加NaHCO₃培养6天后的组织学检查显示仅存在散在的纤毛团块。与用NaHCO₃缓冲的培养物相比，在HEPES加NaHCO₃缓冲的培养物中，[¹⁴C] - 葡糖胺掺入细胞内糖蛋白的量减少了75%，掺入分泌型糖蛋白的量减少了54%。[³H] - 岩藻糖掺入细胞内糖蛋白的量也减少了，尽管对分泌型糖蛋白未观察到影响。
