兔气管器官培养物分泌的糖蛋白和糖胺聚糖的结构与代谢
Structure and metabolism of glycoproteins and glycosaminoglycans secreted by organ cultures of rabbit trachea.
作者信息
Gallagher J T, Kent P W
出版信息
Biochem J. 1975 May;148(2):187-96. doi: 10.1042/bj1480187.
Abstract
- When cultured in medium 199 in an atmosphere of O2+CO2 (95:5) rabbit tracheal explants retained their viability for up to 21 days. 2. The explants secreted into the culture medium three electrophoretically separable components which were eluted in the non-retarded fraction from Sephadex G-200. 3. Digestion with papain and fractionation with a LiCl gradient on DEAE-cellulose resulted in the separation of these components, which were identified as a sialic acid-rich glycoprotein of epithelial origin, and chondroitin sulphate and hyaluronic acid from sub-epithelial cartilage and connective tissue. 4. Incorporation of radioactive precursors ( D-[U-14-C]glucose, D-[1-14-C]glucosamine, D-[1-14-C]galactose and Na2-35SO4) into these secreted macromolecules was indicative of their active biosynthesis by the tracheal tissue.
摘要
- 在含95%氧气和5%二氧化碳的混合气体环境中，于199培养基中培养时，兔气管外植体可保持活力长达21天。2. 外植体向培养基中分泌了三种可通过电泳分离的成分，这些成分在Sephadex G - 200的无滞留组分中被洗脱出来。3. 用木瓜蛋白酶消化并用DEAE - 纤维素上的LiCl梯度进行分级分离，导致这些成分被分离，它们被鉴定为上皮来源的富含唾液酸的糖蛋白，以及来自上皮下软骨和结缔组织的硫酸软骨素和透明质酸。4. 将放射性前体（D - [U - 14 - C]葡萄糖、D - [1 - 14 - C]葡糖胺、D - [1 - 14 - C]半乳糖和Na2 - 35SO4）掺入这些分泌的大分子中，表明气管组织对它们进行了活跃的生物合成。
相似文献
1
Structure and metabolism of glycoproteins and glycosaminoglycans secreted by organ cultures of rabbit trachea.兔气管器官培养物分泌的糖蛋白和糖胺聚糖的结构与代谢
Biochem J. 1975 May;148(2):187-96. doi: 10.1042/bj1480187.
2
Glycoprotein biosynthesis by organ cultures of hamster trachea.仓鼠气管器官培养物的糖蛋白生物合成
Biochim Biophys Acta. 1976 Nov 18;451(1):184-200. doi: 10.1016/0304-4165(76)90270-1.
3
Biosynthesis of respiratory-tract mucins. Incorporation of radioactive precursors into glycoproteins by canine tracheal explants in vitro.呼吸道黏蛋白的生物合成。犬气管外植体在体外将放射性前体掺入糖蛋白的过程。
Biochem J. 1973 Dec;136(4):837-44. doi: 10.1042/bj1360837.
4
Metabolism of respiratory tissue: secretion of mucosubstances by organ cultures of mammalian trachea.呼吸组织的代谢：哺乳动物气管器官培养物中粘液物质的分泌。
Biochem J. 1971 Oct;124(5):59P-60P. doi: 10.1042/bj1240059pb.
5
The synthesis of glycosaminoglycans by cultures of rabbit corneal endothelial and stromal cells.兔角膜内皮细胞和基质细胞培养物对糖胺聚糖的合成
Biochem J. 1976 Sep 15;158(3):567-73. doi: 10.1042/bj1580567.
6
Biosynthesis of proteoglycan in vitro by cartilage from human osteochondrophytic spurs.人骨软骨赘中软骨体外蛋白聚糖的生物合成
Biochem J. 1982 Aug 15;206(2):329-41. doi: 10.1042/bj2060329.
7
8
Mucus glycoprotein secretion by tracheal explants: effects of pollutants.气管外植体的黏液糖蛋白分泌：污染物的影响。
Environ Health Perspect. 1980 Apr;35:131-7. doi: 10.1289/ehp.8035131.
9
Incorporation of N-fluoroacetyl-D-glucosamine into hyaluronate by rabbit tracheal explants in organ culture.兔气管外植体在器官培养中将N-氟乙酰-D-葡萄糖胺掺入透明质酸中。
Biochem J. 1979 Sep 15;182(3):707-16. doi: 10.1042/bj1820707.
10
Biosynthesis of glycosaminoglycans by trabecular meshwork cells in vitro.小梁网细胞在体外对糖胺聚糖的生物合成
Curr Eye Res. 1987 Aug;6(8):959-67. doi: 10.3109/02713688709034867.
引用本文的文献
1
Mucus glycoprotein secretion by tracheal explants: effects of pollutants.气管外植体的黏液糖蛋白分泌：污染物的影响。
Environ Health Perspect. 1980 Apr;35:131-7. doi: 10.1289/ehp.8035131.
2
Glycoprotein secretion by cultured hamster trachea epithelial cells: a model system for in vitro studies of mucus synthesis.培养的仓鼠气管上皮细胞的糖蛋白分泌：用于黏液合成体外研究的模型系统。
In Vitro. 1980 Apr;16(4):320-9. doi: 10.1007/BF02618338.
3
Physicochemical properties of avian tracheal mucus.禽气管黏液的物理化学性质。
Biochem J. 1982 Mar 1;201(3):533-42. doi: 10.1042/bj2010533.
4
Regulation of the synthesis of mucin glycoproteins in swine trachea explants.猪气管外植体中黏蛋白糖蛋白合成的调控
In Vitro. 1984 May;20(5):416-32. doi: 10.1007/BF02619588.
5
Biochemical studies of the tracheobronchial epithelium.气管支气管上皮的生化研究。
Environ Health Perspect. 1984 Jun;56:61-74. doi: 10.1289/ehp.845661.
6
Comparison of mucus flow rate, radiolabelled glycoprotein output and smooth muscle contraction in the ferret trachea in vitro.雪貂气管体外黏液流速、放射性标记糖蛋白输出及平滑肌收缩的比较
Br J Pharmacol. 1988 Jun;94(2):293-8. doi: 10.1111/j.1476-5381.1988.tb11530.x.
7
Mucin-like glycoprotein secreted by cultured hamster tracheal epithelial cells. Biochemical and immunological characterization.培养的仓鼠气管上皮细胞分泌的黏蛋白样糖蛋白。生化及免疫学特性
Biochem J. 1991 Aug 1;277 ( Pt 3)(Pt 3):713-8. doi: 10.1042/bj2770713.
8
Incorporation of N-fluoroacetyl-D-glucosamine into hyaluronate by rabbit tracheal explants in organ culture.兔气管外植体在器官培养中将N-氟乙酰-D-葡萄糖胺掺入透明质酸中。
Biochem J. 1979 Sep 15;182(3):707-16. doi: 10.1042/bj1820707.
本文引用的文献
1
Biosynthesis of intestinal mucins. 4. Utilization of [1-C]glucose by sheep colonic mucosa in vitro.肠道黏蛋白的生物合成。4. 绵羊结肠黏膜在体外对[1-C]葡萄糖的利用。
Biochem J. 1963 Feb;86(2):248-54. doi: 10.1042/bj0860248.
2
The determination of chondroitin sulfate C-type polysaccharides in mixtures with other acid mucopolysaccharides.与其他酸性粘多糖混合存在时硫酸软骨素C型多糖的测定
Biochim Biophys Acta. 1961 Nov 11;53:509-13. doi: 10.1016/0006-3002(61)90209-8.
3
Aliphatic ammonium salts in the assay of acidic polysaccharides from tissues.组织中酸性多糖测定中的脂肪族铵盐
Methods Biochem Anal. 1960;8:145-97. doi: 10.1002/9780470110249.ch4.
4
5
ON THE FORMATION OF MUCIN IN TRACHEAL MUCOSA OF THE RAT.大鼠气管黏膜中黏蛋白的形成
Biochim Biophys Acta. 1964 Jul 7;83:239-41. doi: 10.1016/0926-6526(64)90042-4.
6
THE DETERMINATION OF SMALL QUANTITIES OF ISOMERIC CHONDROITIN SULFATES.
Biochim Biophys Acta. 1964 Mar 2;83:137-40. doi: 10.1016/0926-6526(64)90065-5.
7
Fractionation of acid mucopolysaccharides on DEAE-Sephadex anion exchanger.酸性粘多糖在DEAE-葡聚糖阴离子交换剂上的分级分离
Biochim Biophys Acta. 1962 Sep 24;63:346-8. doi: 10.1016/0006-3002(62)90692-3.
8
A modified uronic acid carbazole reaction.一种改良的糖醛酸咔唑反应。
Anal Biochem. 1962 Oct;4:330-4. doi: 10.1016/0003-2697(62)90095-7.
9
Determination of maltase and isomaltase activities with a glucose-oxidase reagent.用葡萄糖氧化酶试剂测定麦芽糖酶和异麦芽糖酶活性。
Biochem J. 1961 Sep;80(3):547-51. doi: 10.1042/bj0800547.
10
The thiobarbituric acid assay of sialic acids.唾液酸的硫代巴比妥酸测定法。
J Biol Chem. 1959 Aug;234(8):1971-5.
Zotero 插件