兔气管器官培养物分泌的糖蛋白和糖胺聚糖的结构与代谢
Structure and metabolism of glycoproteins and glycosaminoglycans secreted by organ cultures of rabbit trachea.
作者信息
Gallagher J T, Kent P W
出版信息
Biochem J. 1975 May;148(2):187-96. doi: 10.1042/bj1480187.
Abstract
- When cultured in medium 199 in an atmosphere of O2+CO2 (95:5) rabbit tracheal explants retained their viability for up to 21 days. 2. The explants secreted into the culture medium three electrophoretically separable components which were eluted in the non-retarded fraction from Sephadex G-200. 3. Digestion with papain and fractionation with a LiCl gradient on DEAE-cellulose resulted in the separation of these components, which were identified as a sialic acid-rich glycoprotein of epithelial origin, and chondroitin sulphate and hyaluronic acid from sub-epithelial cartilage and connective tissue. 4. Incorporation of radioactive precursors ( D-[U-14-C]glucose, D-[1-14-C]glucosamine, D-[1-14-C]galactose and Na2-35SO4) into these secreted macromolecules was indicative of their active biosynthesis by the tracheal tissue.
摘要
- 在含95%氧气和5%二氧化碳的混合气体环境中，于199培养基中培养时，兔气管外植体可保持活力长达21天。2. 外植体向培养基中分泌了三种可通过电泳分离的成分，这些成分在Sephadex G - 200的无滞留组分中被洗脱出来。3. 用木瓜蛋白酶消化并用DEAE - 纤维素上的LiCl梯度进行分级分离，导致这些成分被分离，它们被鉴定为上皮来源的富含唾液酸的糖蛋白，以及来自上皮下软骨和结缔组织的硫酸软骨素和透明质酸。4. 将放射性前体（D - [U - 14 - C]葡萄糖、D - [1 - 14 - C]葡糖胺、D - [1 - 14 - C]半乳糖和Na2 - 35SO4）掺入这些分泌的大分子中，表明气管组织对它们进行了活跃的生物合成。
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