McClellan Kathy, Howard Kevin, Mayhew Elizabeth, Niederkorn Jerry, Alizadeh Hassan
Department of Ophthalmology, University of Sydney, GPO BOX 4337, Sydney, NSW 2121, Australia.
Exp Eye Res. 2002 Sep;75(3):285-93.
Acanthamoeba cysts are not eliminated from the corneas of human subjects or experimentally infected animals. The persistence of Acanthamoeba cysts in the cornea indicates that either the cysts escape immunological elimination or are not recognized by the host's immunological elements. The aim of this study was to determine the immunogenicity and antigenicity of the Acanthamoeba cyst. Mice were immunized intraperitoneally and serum anti-Acanthamoeba IgG was measured by ELISA. Lymphoproliferative assay and delayed type hypersensitivity (DTH) responses to Acanthamoeba castellanii cyst and trophozoite antigens were used to determine the cell mediated immune responses against Acanthamoeba cysts. A. castellanii cysts were both immunogenic and antigenic, producing anti-Acanthamoeba serum IgG, T lymphocyte proliferation, and delayed type hypersensitivity responses. These results indicate that Acanthamoeba cysts are recognized by the immune system. The persistence of the organism in the human cornea means that these adaptive immune responses fail to kill Acanthamoeba cysts.
棘阿米巴包囊无法从人类受试者或实验感染动物的角膜中清除。棘阿米巴包囊在角膜中的持续存在表明，要么包囊逃避了免疫清除，要么未被宿主的免疫成分识别。本研究的目的是确定棘阿米巴包囊的免疫原性和抗原性。通过腹腔注射对小鼠进行免疫，并采用酶联免疫吸附测定法（ELISA）检测血清抗棘阿米巴IgG。利用淋巴细胞增殖试验和对卡氏棘阿米巴包囊及滋养体抗原的迟发型超敏反应（DTH）来确定针对棘阿米巴包囊的细胞介导免疫反应。卡氏棘阿米巴包囊具有免疫原性和抗原性，可产生抗棘阿米巴血清IgG、T淋巴细胞增殖以及迟发型超敏反应。这些结果表明棘阿米巴包囊可被免疫系统识别。该生物体在人角膜中的持续存在意味着这些适应性免疫反应无法杀死棘阿米巴包囊。
