Saito Masahiro, Kiyokawa Nobutaka, Taguchi Tomoko, Suzuki Kyoko, Sekino Takaomi, Mimori Kenichi, Suzuki Toyo, Nakajima Hideki, Katagiri Yohko U, Fujimura Junya, Fujita Hiroo, Ishimoto Koichi, Yamashiro Yuichiro, Fujimoto Junichiro
Department of Developmental Biology, National Research Institute for Child Health and Development, Tokyo, Japan.
Exp Hematol. 2002 Oct;30(10):1115-23. doi: 10.1016/s0301-472x(02)00889-5.
Recent reports have indicated that monocytes express receptors for the granulocyte colony-stimulating factor (G-CSF). The direct effects of G-CSF on cytokine secretion in monocytes were examined.
A monocytic cell line NOMO-1 that secretes multiple cytokines upon stimulation with lipopolysaccharide (LPS) was used. Normal human monocytes were purified by negative selection using magnetic beads. Cells pretreated with or without G-CSF were stimulated with LPS, and the subsequent concentrations of cytokines and chemokines in supernatants were determined by sandwich enzyme-linked immunosorbent assay.
NOMO-1 cells were found to express receptors for G-CSF. Although G-CSF stimulation did not induce cytokine secretion, pretreatment with G-CSF significantly attenuated LPS-stimulated secretion of the proinflammatory cytokines tumor necrosis factor-alpha and interleukin (IL)-12 in NOMO-1 cells. Simultaneously, however, G-CSF pretreatment apparently enhanced LPS-induced secretion of IL-10 and monocyte chemoattractant protein-1, whereas secretions of IL-1beta, IL-6, and IL-8 were unaffected. When normal human monocytes from healthy volunteers were similarly examined, marked individual variations in LPS-induced secretion of cytokines were observed. Although some exceptions exist, a similar tendency as to the effects of G-CSF treatment on cytokine secretions as that in NOMO-1 cells was observed in human monocytes.
Our data suggest that G-CSF directly affects monocytes and modulates their cytokine secretion. NOMO-1 cells can provide an alternate model for in vitro culture of monocytes to investigate the effects of G-CSF on cytokine secretion by these cells.
最近的报告表明单核细胞表达粒细胞集落刺激因子（G-CSF）受体。研究了G-CSF对单核细胞细胞因子分泌的直接影响。
使用单核细胞系NOMO-1，该细胞系在脂多糖（LPS）刺激下分泌多种细胞因子。通过磁珠阴性选择纯化正常人单核细胞。用LPS刺激经或未经G-CSF预处理的细胞，通过夹心酶联免疫吸附测定法测定上清液中随后的细胞因子和趋化因子浓度。
发现NOMO-1细胞表达G-CSF受体。虽然G-CSF刺激未诱导细胞因子分泌，但G-CSF预处理显著减弱了NOMO-1细胞中LPS刺激的促炎细胞因子肿瘤坏死因子-α和白细胞介素（IL）-12的分泌。然而，同时，G-CSF预处理明显增强了LPS诱导的IL-10和单核细胞趋化蛋白-1的分泌，而IL-1β、IL-6和IL-8的分泌未受影响。当对健康志愿者的正常人单核细胞进行类似检查时，观察到LPS诱导的细胞因子分泌存在明显的个体差异。尽管存在一些例外情况，但在人单核细胞中观察到与NOMO-1细胞中G-CSF处理对细胞因子分泌的影响相似的趋势。
我们的数据表明G-CSF直接影响单核细胞并调节其细胞因子分泌。NOMO-1细胞可为体外培养单核细胞提供替代模型，以研究G-CSF对这些细胞细胞因子分泌的影响。
