Choi Sun Ju, Park Joo Young, Lee Young Kyu, Choi Hyun Il, Lee Young Sim, Koh Choon-Myung, Chung In-Bai
Department of Microbiology, Institute of Basic Medical Science, Yonsei University, Wonju College of Medicine, Kangwon-do, Korea.
Am J Reprod Immunol. 2002 Aug;48(2):70-6. doi: 10.1034/j.1600-0897.2002.01071.x.
The mechanism through which vascular endothelial growth factor (VEGF) regulation occurs at the feto-maternal interface is poorly understood. The aim of this study was to investigate the effects of various cytokines on VEGF expression and secretion by trophoblast cells.
We investigated the effects of cytokines on VEGF expression in human first trimester trophoblast cell line by analyzing VEGF messenger RNA (mRNA) by reverse transcription-polymerase chain reaction and VEGF protein secretion by enzyme linked immunosorbent assay.
The trophoblast cells expressed VEGF mRNA constitutively and the main subtypes were identified as VEGF121 and VEGF165. When cultured in the presence of interferon (IFN)-gamma, interleukin (IL)- 1beta, tumor necrosis factor (TNF)-alpha, IL-2, or IL-10, VEGF mRNA expression was found to be significantly increased by IL-1beta, IFN-gamma and TNF-alpha but to be unaffected by IL-2 and IL-10. Moreover, VEGF secretion was most significantly increased by IFN-gamma treatment.
These results suggest that IL-1beta, IFN-gamma, and TNF-alpha may regulate the production of VEGF in early gestational trophoblasts.
血管内皮生长因子（VEGF）在母胎界面处的调控机制尚不清楚。本研究旨在探讨多种细胞因子对滋养层细胞VEGF表达和分泌的影响。
我们通过逆转录-聚合酶链反应分析VEGF信使核糖核酸（mRNA）以及采用酶联免疫吸附测定法检测VEGF蛋白分泌，研究细胞因子对人早孕滋养层细胞系中VEGF表达的影响。
滋养层细胞组成性表达VEGF mRNA，主要亚型鉴定为VEGF121和VEGF165。当在干扰素（IFN）-γ、白细胞介素（IL）-1β、肿瘤坏死因子（TNF）-α、IL-2或IL-10存在的情况下进行培养时，发现IL-1β、IFN-γ和TNF-α可显著增加VEGF mRNA表达，而IL-2和IL-10对其无影响。此外，IFN-γ处理可使VEGF分泌增加最为显著。
这些结果表明，IL-1β、IFN-γ和TNF-α可能调控早孕滋养层细胞中VEGF的产生。
