Bennett W A, Lagoo-Deenadayalan S, Brackin M N, Hale E, Cowan B D
Department of Obstetrics and Gynecology, University of Mississippi Medical Center, Jackson, USA.
Am J Reprod Immunol. 1996 Nov;36(5):285-94. doi: 10.1111/j.1600-0897.1996.tb00178.x.
Cytokines form an important communication network between the mother and fetus. Defining the significance of these factors requires an understanding of their constitutive expression by maternal and fetal tissues. This study examines cytokine expression by human trophoblast.
A reverse transcription polymerase chain reaction (RT-PCR) technique was used to assess cytokine expression by choriocarcinoma cells (BeWo, JEG-3, and JAR) and term trophoblast. Placental digests were enriched for trophoblast by immunoaffinity (CD-9) columns.
Interleukin (IL)-1, tumor necrosis factor (TNF)-alpha and interferon (IFN)-gamma were weakly expressed or absent in the choriocarcinoma cells. In contrast, these cytokines were expressed by term trophoblast. IL-6, IL-10, IFN-alpha, IFN-beta, and granulocyte macrophage-colony stimulating factor (GM-CSF) mRNA were detected in all trophoblast cells, except for a paucity of IL-10 expression by JEG-3 cells.
Human choriocarcinoma cells and term trophoblast express cytokines that may regulate critical reproductive events. Expression of inflammatory cytokines such as IL-1, TNF-alpha, and IFN-gamma by term trophoblast could trigger labor or be a consequence of labor-associated events.
细胞因子构成了母胎之间重要的通讯网络。明确这些因子的重要性需要了解母体和胎儿组织对它们的组成性表达。本研究检测了人滋养层细胞的细胞因子表达。
采用逆转录聚合酶链反应（RT-PCR）技术评估绒毛膜癌细胞（BeWo、JEG-3和JAR）和足月滋养层细胞的细胞因子表达。通过免疫亲和（CD-9）柱富集胎盘消化物中的滋养层细胞。
白细胞介素（IL）-1、肿瘤坏死因子（TNF）-α和干扰素（IFN）-γ在绒毛膜癌细胞中表达较弱或未表达。相比之下，这些细胞因子在足月滋养层细胞中表达。在所有滋养层细胞中均检测到IL-6、IL-10、IFN-α、IFN-β和粒细胞巨噬细胞集落刺激因子（GM-CSF）的mRNA，但JEG-3细胞中IL-10表达较少。
人绒毛膜癌细胞和足月滋养层细胞表达的细胞因子可能调节关键的生殖事件。足月滋养层细胞表达的IL-1、TNF-α和IFN-γ等炎性细胞因子可能引发分娩，或是与分娩相关事件的结果。
