Abiko Yoshihiro, Jinbu Yoshinori, Noguchi Tadahide, Nishimura Michiko, Kusano Kaoru, Amaratunga Prasad, Shibata Toshiyuki, Kaku Tohru
Department of Oral Pathology, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan.
Pathol Res Pract. 2002;198(8):537-42. doi: 10.1078/0344-0338-00298.
Human beta defensin 2 (hBD-2) is a major antimicrobial peptide that is produced by many types of epithelial cells, and is transcriptionally inducible by various proinflammatory agents, such as cytokines and bacteria. Although in vitro studies of the hBDs in oral epithelial cells have been well documented, only little is known about the in vivo pathological state of oral epithelium. We investigated the localization of hBD-2 peptide in tissue sections of oral lichen planus, leukoplakia, candidal leukoplakia and radicular cysts using immunohistochemistry. HBD-2 was stained in both the hyperkeratinized and the granular layers in cases of lichen planus with hyperkeratosis and leukoplakia. Expression in spinous and suprabasal layers was often strong in lichen planus. There were no significant differences in the number of S-100 positive dendritic cells between the widely stained areas and those with limited staining areas in lichen planus. In cases of candidal leukoplakia, the hyphae of candida were mainly detected on the surface of keratinization, which showed only negative or faint staining for hBD-2. These results suggest that hBD-2 is vigorously induced by lichen planus-related inflammation and that it plays an important role in protection from Candida albicans infection; however, it is not a strong chemotactic attractant for Langerhans cells in pathological conditions of oral epithelium.
人β-防御素2（hBD-2）是一种主要的抗菌肽，由多种上皮细胞产生，并且可被多种促炎因子如细胞因子和细菌转录诱导。虽然关于口腔上皮细胞中hBDs的体外研究已有充分记载，但对于口腔上皮的体内病理状态却知之甚少。我们使用免疫组织化学方法研究了hBD-2肽在口腔扁平苔藓、白斑、念珠菌性白斑和根端囊肿组织切片中的定位。在伴有角化过度的扁平苔藓和白斑病例中，hBD-2在角化过度层和颗粒层均有染色。扁平苔藓棘层和基底层上方的表达通常较强。扁平苔藓中广泛染色区域和染色受限区域之间S-100阳性树突状细胞的数量没有显著差异。在念珠菌性白斑病例中，念珠菌的菌丝主要在角化表面被检测到，此处hBD-2仅呈阴性或弱阳性染色。这些结果表明，hBD-2被扁平苔藓相关炎症强烈诱导，并且在抵御白色念珠菌感染中发挥重要作用；然而，在口腔上皮的病理状态下，它对朗格汉斯细胞不是一种强大的趋化吸引剂。
