Yang Jun, Ding Zhen-Hua, Luo Cheng-Ji, He Pei, Zheng Li
Department of Teaching Affairs, First Military Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2002 May;22(5):406-8.
To study the apoptotic patterns of bone marrow stromal cells (BMSCs) after irradiation.
Apoptosis of BMSCs induced by (60)Co gamma irradiation was detected by both flow cytometry and electron microscopy.
Compared with normal control group, no manifest changes in the apoptosis ratio occurred in BMSCs after 50 Gy irradiation (P>0.05) as demonstrated by flow cytometry. Four hours after 100 Gy irradiation, manifest changes in apoptosis ratio of BMSCs were observed (P<0.01), reaching the peak 20 h later. Similar apoptotic patterns occurred in BMSCs exposed to irradiation of 200 Gy (P>0.05), only the apoptosis ratio was slightly higher.
Irradiation of proper dose may induce apoptosis in vitro in BMSCs that possess strong resistance to irradiation.
研究照射后骨髓基质细胞（BMSCs）的凋亡模式。
采用流式细胞术和电子显微镜检测（60）Coγ射线照射诱导的BMSCs凋亡。
流式细胞术检测结果显示，与正常对照组相比，50 Gy照射后BMSCs的凋亡率无明显变化（P>0.05）。100 Gy照射4小时后，BMSCs的凋亡率出现明显变化（P<0.01），20小时后达到峰值。200 Gy照射的BMSCs出现类似的凋亡模式（P>0.05），只是凋亡率略高。
适当剂量的照射可在体外诱导对辐射具有较强抗性的BMSCs发生凋亡。
