Lin Hui-liang, Yang Lan, Wang Xin-rong, Gu Hong-tao, Gao Guang-xun
Department of Hematology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007 Sep;23(9):813-5.
To study the effect of platelet factor 4 (PF4) on murine bone marrow cell apoptosis after irradiation with 5.0 Gy (60)COgamma and explore its potential mechanism.
30 male BALB/c mice were randomly divided into three groups: namely normal control group (n = 6), PF4 treatment group (n=12) and irradiation (IR) group (n = 12). The mice in PF4 treatment group were administrated intraperitoneally PF4 40 microg/kg at 26 h and 20 h respectively before total-body irradiation with 5.0 Gy (60)COgamma ray. At 4 h and 20 h after irradiation the apoptosis of bone marrow cells were detected by flow cytometry (FCM) and the expression of Bax protein was identified by Western blot.
At 4 h and 20 h after irradiation the apoptosis of bone marrow cells in PF4 treatment group was significantly lower than that in IR group (P < 0.01), the expression of Bax protein in the PF4 group was lower than that in the IR group (P < 0.05).
PF4 can protect bone marrow cells from radiation-induced apoptosis, which is partly due to the down-regulation of proapoptotic protein Bax expression.
研究血小板因子4（PF4）对5.0 Gy （60）Coγ射线照射后小鼠骨髓细胞凋亡的影响，并探讨其潜在机制。
将30只雄性BALB/c小鼠随机分为三组：正常对照组（n = 6）、PF4治疗组（n = 12）和照射（IR）组（n = 12）。PF4治疗组小鼠在全身照射5.0 Gy （60）Coγ射线前26 h和20 h分别腹腔注射PF4 40 μg/kg。照射后4 h和20 h，采用流式细胞术（FCM）检测骨髓细胞凋亡情况，采用蛋白质免疫印迹法检测Bax蛋白表达。
照射后4 h和20 h，PF4治疗组骨髓细胞凋亡率明显低于IR组（P < 0.01），PF4组Bax蛋白表达低于IR组（P < 0.05）。
PF4可保护骨髓细胞免受辐射诱导的凋亡，部分原因是促凋亡蛋白Bax表达下调。
