酰基辅酶A结合蛋白是哺乳动物细胞系中的一种必需蛋白。
Acyl-CoA binding protein is an essential protein in mammalian cell lines.
作者信息
Faergeman Nils J, Knudsen Jens
机构信息
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.
出版信息
Biochem J. 2002 Dec 15;368(Pt 3):679-82. doi: 10.1042/BJ20021413.
Abstract
In the present work, small interference RNA was used to knock-down acyl-CoA binding protein (ACBP) in HeLa, HepG2 and Chang cells. Transfection with ACBP-specific siRNA stopped growth, detached cells from the growth surface and blocked thymidine and acetate incorporation. The results show that depletion of ACBP in mammalian cells results in lethality, suggesting that ACBP is an essential protein.
摘要
在本研究中，利用小干扰RNA敲低HeLa细胞、HepG2细胞和Chang细胞中的酰基辅酶A结合蛋白（ACBP）。用ACBP特异性小干扰RNA转染会使细胞停止生长，使其从生长表面脱离，并阻止胸苷和乙酸盐的掺入。结果表明，哺乳动物细胞中ACBP的缺失会导致细胞死亡，这表明ACBP是一种必需蛋白。
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