Wang Da-Zhi, Li Shijie, Hockemeyer Dirk, Sutherland Lillian, Wang Zhigao, Schratt Gerhard, Richardson James A, Nordheim Alfred, Olson Eric N
Department of Molecular Biology, University of Texas, Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas 75390-9148, USA.
Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14855-60. doi: 10.1073/pnas.222561499. Epub 2002 Oct 23.
Myocardin is a SAP (SAF-A/B, Acinus, PIAS) domain transcription factor that associates with serum response factor (SRF) to potently enhance SRF-dependent transcription. Here we describe two myocardin-related transcription factors (MRTFs), A and B, that also interact with SRF and stimulate its transcriptional activity. Whereas myocardin is expressed specifically in cardiac and smooth muscle cells, MRTF-A and -B are expressed in numerous embryonic and adult tissues. In SRF-deficient embryonic stem cells, myocardin and MRTFs are unable to activate SRF-dependent reporter genes, confirming their dependence on SRF. Myocardin and MRTFs comprise a previously uncharacterized family of SRF cofactors with the potential to modulate SRF target genes in a wide range of tissues.
心肌素是一种SAP（SAF-A/B、Acinus、PIAS）结构域转录因子，它与血清反应因子（SRF）结合，有力地增强SRF依赖的转录。在此，我们描述了两种心肌素相关转录因子（MRTF），即A和B，它们也与SRF相互作用并刺激其转录活性。心肌素特异性表达于心肌和平滑肌细胞，而MRTF-A和 -B则在许多胚胎和成年组织中表达。在缺乏SRF的胚胎干细胞中，心肌素和MRTF无法激活SRF依赖的报告基因，证实了它们对SRF的依赖性。心肌素和MRTF构成了一个以前未被描述的SRF辅因子家族，有可能在广泛的组织中调节SRF靶基因。
