[血浆成分在由普洛沙诺268稳定的氟碳乳剂颗粒表面的吸附作用]

[Sorption of plasma components on the surface of particles of fluorocarbon emulsions stabilized by proxanol 268].

作者信息

Sklifas A N, Shekhtman D G, Evdokimov V A, Temnov A A, Antashov A V, Kaptsov V V, Ponomarchuk V V, Vorob'ev S I, Kukushkin N I

机构信息

Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.

出版信息

Biofizika. 2002 Sep-Oct;47(5):926-32.

PMID:12397968

Abstract

It was found in the experiments in vivo and in vitro that the contact of perfluorocarbon emulsion stabilized with proxanol 268 with blood plasma leads to the sorption of various plasma proteins on the surface of emulsion particles. The profile of the proteins sorbed is complex and includes proteins with molecular weights ranging from 14 to 94 kDa. Among proteins sorbed on the emulsion particles circulating in blood, IgG was identified. Incubation of the emulsion stabilized with proxanol 268 with human blood plasma in vitro was shown to result in the sorption of IgG and IgA the perfluorocarbon particles. The sorbtion of serum proteins and immune complexes circulating in blood on the surface of perfluorocarbon particles stabilized with proxanol 268 was revealed to activate the complement system.

摘要

体内和体外实验均发现，用普洛醇268稳定的全氟碳乳液与血浆接触会导致各种血浆蛋白吸附在乳液颗粒表面。吸附的蛋白质谱很复杂，包括分子量在14至94 kDa之间的蛋白质。在血液中循环的乳液颗粒上吸附的蛋白质中，鉴定出了IgG。体外实验表明，用普洛醇268稳定的乳液与人血浆孵育会导致IgG和IgA吸附到全氟碳颗粒上。还发现，血液中循环的血清蛋白和免疫复合物吸附在用普洛醇268稳定的全氟碳颗粒表面会激活补体系统。