Lautenschlager Irmeli, Härmä Maiju, Höckerstedt Krister, Linnavuori Kimmo, Loginov Raisa, Taskinen Eero
Department of Virology, Helsinki University Central Hospital and University of Helsinki, Haartmaninkatu 3, FIN-00290 Helsinki, Finland.
J Hepatol. 2002 Nov;37(5):648-54. doi: 10.1016/s0168-8278(02)00246-5.
BACKGROUND/AIMS: Human herpesvirus-6 (HHV-6) infection has been recently described in liver transplants. HHV-6 may infect the transplant and cause graft dysfunction. Some association between HHV-6 and rejection has also been recorded. We have now investigated the possible involvement of HHV-6 in the intragraft immunological processes, adhesion molecules induction and lymphocyte activation.
HHV-6 was detected in liver biopsies of 19 patients transplanted in the period from 1996 to 2000. Patients with other infections or rejection were excluded from the study. Finally, 19 biopsies of eight allografts with pure HHV-6 infection were available. Adhesion molecules (ICAM-1, VCAM-1, ELAM-1) and their ligands (LFA-1, VLA-4, sLeX) and lymphoid activation markers (MHC class II, IL-2R) were demonstrated in liver biopsies by immunohistochemistry. Five biopsies from patients with normal graft function and without rejection or infection were used as controls for immune staining, and ten biopsies with acute rejection but without infection were used as positive controls.
Biopsy histology demonstrated mild to moderate lymphocyte infiltration associated with HHV-6 infection. HHV-6 significantly (P < or = 0.05) increased the vascular expression of ICAM-1 and VCAM-1, and the number of graft infiltrating lymphocytes positive for LFA-1, VLA-4 and class II antigens. A total of 3/8 grafts developed chronic rejection.
HHV-6 infection increased adhesion molecule expression and lymphocyte infiltration in liver allografts.
背景/目的：最近在肝移植中发现了人疱疹病毒6型（HHV-6）感染。HHV-6可能感染移植物并导致移植物功能障碍。也有记录显示HHV-6与排斥反应之间存在某种关联。我们现在研究了HHV-6在移植物内免疫过程、黏附分子诱导和淋巴细胞激活中可能的作用。
在1996年至2000年期间对19例接受移植的患者的肝活检组织进行HHV-6检测。将患有其他感染或排斥反应的患者排除在研究之外。最后，获得了8个同种异体移植物中单纯HHV-6感染的19份活检组织。通过免疫组织化学在肝活检组织中检测黏附分子（ICAM-1、VCAM-1、ELAM-1）及其配体（LFA-1、VLA-4、sLeX）和淋巴细胞激活标志物（MHC II类、IL-2R）。将5例移植物功能正常且无排斥反应或感染的患者的活检组织用作免疫染色对照，将10例有急性排斥反应但无感染的活检组织用作阳性对照。
活检组织学显示与HHV-6感染相关的轻度至中度淋巴细胞浸润。HHV-6显著（P≤0.05）增加了ICAM-1和VCAM-1的血管表达，以及LFA-1、VLA-4和II类抗原阳性的移植物浸润淋巴细胞数量。总共3/8的移植物发生了慢性排斥反应。
HHV-6感染增加了肝同种异体移植物中黏附分子的表达和淋巴细胞浸润。
