Lautenschlager I, Höckerstedt K, Taskinen E, von Willebrand E
Laboratory Department, Helsinki University Central Hospital, University of Helsinki, Finland.
Transpl Int. 1996;9 Suppl 1:S213-5. doi: 10.1007/978-3-662-00818-8_54.
Vascular adhesion molecules and their ligands are important in leukocyte-endothelial cell interactions and in T-cell activation of rejection cascade. Also, cytomegalovirus (CMV) infection is suggested to be involved in the mechanisms of rejection. In this study, the expression of vascular adhesion molecules ICAM-1, VCAM-1 and ELAM-1 in the liver allografts, the number of leukocytes positive for their ligands LFA-1, VLA-4 and SLex, and activation markers (class II, IL2-receptor) were investigated in liver allografts during CMV infection and acute rejection and compared to grafts with normal function and histology. The adhesion molecules, their ligands and activation markers were demonstrated from liver biopsy frozen sections by the immunoperoxidase technique and monoclonal antibodies. A significant induction of ICAM-1 and VCAM-1 was seen in vascular and sinusoidal endothelium associated with both CMV and rejection, and induction of ELAM-1 in vascular endothelium in rejection only. In both cases, the number of leukocytes expressing LFA-1 was significantly increased, but VLA-4-positive cells were more characteristic for CMV and SLex-positive cells more for rejection. IL2-receptor positivity was practically seen in rejection only, but class II-expressing cells were increased during both CMV infection and rejection. In conclusion, adhesion molecules were induced and the infiltrating cells expressed their ligands both in liver rejection and during CMV infection, although the expression pattern was slightly different.
血管黏附分子及其配体在白细胞与内皮细胞相互作用以及排斥反应级联的T细胞激活过程中起着重要作用。此外，有迹象表明巨细胞病毒（CMV）感染参与了排斥反应机制。在本研究中，对CMV感染和急性排斥反应期间肝移植中血管黏附分子ICAM-1、VCAM-1和ELAM-1的表达、其配体LFA-1、VLA-4和SLex阳性的白细胞数量以及激活标志物（II类、IL-2受体）进行了研究，并与功能和组织学正常的移植物进行了比较。通过免疫过氧化物酶技术和单克隆抗体从肝活检冰冻切片中检测黏附分子、其配体和激活标志物。在与CMV和排斥反应相关的血管和窦状内皮中均可见ICAM-1和VCAM-1的显著诱导，而ELAM-1仅在排斥反应的血管内皮中诱导。在这两种情况下，表达LFA-1的白细胞数量均显著增加，但VLA-4阳性细胞在CMV感染中更具特征性，而SLex阳性细胞在排斥反应中更具特征性。IL-2受体阳性实际上仅在排斥反应中可见，但在CMV感染和排斥反应期间，表达II类分子的细胞均增加。总之，尽管表达模式略有不同，但在肝移植排斥反应和CMV感染期间均诱导了黏附分子，且浸润细胞表达了它们的配体。
