在肠炎沙门氏菌中，嘌呤单核苷酸和组氨酸生物合成途径中的代谢通量都可能影响硫胺素羟甲基嘧啶部分的合成。
Metabolic flux in both the purine mononucleotide and histidine biosynthetic pathways can influence synthesis of the hydroxymethyl pyrimidine moiety of thiamine in Salmonella enterica.
作者信息
Allen Shara, Zilles Julie L, Downs Diana M
机构信息
Department of Bacteriology, University of Wisconsin-Madison, Madison 53706, USA.
出版信息
J Bacteriol. 2002 Nov;184(22):6130-7. doi: 10.1128/JB.184.22.6130-6137.2002.
Abstract
Together, the biosyntheses of histidine, purines, and thiamine pyrophosphate (TPP) contain examples of convergent, divergent, and regulatory pathway integration. Mutations in two purine biosynthetic genes (purI and purH) affect TPP biosynthesis due to flux through the purine and histidine pathways. The molecular genetic characterization of purI mutants and their respective pseudorevertants resulted in the conclusion that <1% of the wild-type activity of the PurI enzyme was sufficient for thiamine but not for purine synthesis. The respective pseudorevertants were found to be informational suppressors. In addition, it was shown that accumulation of the purine intermediate aminoimidazole carboxamide ribotide inhibits thiamine synthesis, specifically affecting the conversion of aminoimidazole ribotide to hydroxymethyl pyrimidine.
摘要
组氨酸、嘌呤和硫胺素焦磷酸（TPP）的生物合成共同包含了汇聚、发散和调节途径整合的例子。两个嘌呤生物合成基因（purI和purH）的突变会影响TPP的生物合成，因为嘌呤和组氨酸途径存在通量。对purI突变体及其各自的假回复体进行分子遗传学表征后得出结论，PurI酶<1%的野生型活性足以合成硫胺素，但不足以合成嘌呤。发现各自的假回复体是信息抑制子。此外，研究表明嘌呤中间体氨基咪唑甲酰胺核糖核苷酸的积累会抑制硫胺素合成，特别影响氨基咪唑核糖核苷酸向羟甲基嘧啶的转化。
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