Huang Hong-Mei, Wang Ke-Min, Yang Rong-Hua, Yang Xiao-Hai, Huang Sha-Sheng, Xiao Dan, Feng Feng
State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, China.
Anal Sci. 2002 Oct;18(10):1111-5. doi: 10.2116/analsci.18.1111.
A new optode membrane for the sensitive determination of berberine based on fluorescence quenching of a conjugated polymer, poly(2,5-dimethoxy-phenyldiacetylene) (PDPA), is proposed. Incorporated in a membrane composed of plasticized poly(vinyl chloride) (PVC), the conjugated polymer exhibits better stability than those small sensing molecules regarding its excellent optical properties and lipophilic characteristics. Moreover, upon the introduction of a negatively charged lipophilic additive (tetraphenylborate salt) into a PVC membrane, the optode displayed enhanced sensitivity. In addition, satisfactory analytical sensing characteristics for determining beberine were obtained in terms of the selectivity, reversibility and reproducibility with a detecting range of between 7.5 x 10(-7) mol l(-1) and 7.5 x 10(-4) mol l(-1). The optode membrane has been applied to determine berberine in commercial tablets. The results showed a good agreement with those obtained by the pharmacopoeial method.
本文提出了一种基于共轭聚合物聚（2,5-二甲氧基-苯基二乙炔）（PDPA）荧光猝灭的新型光极膜，用于灵敏测定黄连素。将该共轭聚合物掺入由增塑聚氯乙烯（PVC）组成的膜中，由于其优异的光学性能和亲脂特性，与那些小分子传感分子相比，它表现出更好的稳定性。此外，在PVC膜中引入带负电荷的亲脂添加剂（四苯基硼酸盐）后，该光极显示出更高的灵敏度。另外，在选择性、可逆性和重现性方面，该光极膜对于测定黄连素具有令人满意的分析传感特性，检测范围在7.5×10⁻⁷ mol·L⁻¹至7.5×10⁻⁴ mol·L⁻¹之间。该光极膜已应用于商业片剂中黄连素的测定。结果表明，其与药典方法所得结果吻合良好。
