Lips K S, Pfeil U, Kummer W
Institute for Anatomy and Cell Biology, Justus-Liebig-University, Aulweg 123, D-35385 Giessen, Germany.
Neuroscience. 2002;115(1):1-5. doi: 10.1016/s0306-4522(02)00274-9.
Previous binding studies have suggested the presence of a so far unknown nicotinic acetylcholine receptor subunit in dorsal root ganglia (Pugh et al., 1995). Here, we investigated whether the most recently identified subunit, alpha10, and its potential interaction partner, alpha9 (Elgoyhen et al., 2001), are expressed in these ganglia. All neurons of rat dorsal root ganglia, but no glial cells, expressed both alpha9 and alpha10 mRNA in in situ hybridization, and exhibited alpha10 immunoreactivity using a newly raised antibody. These findings were confirmed by RT-PCR and western blotting. The data show that dorsal root ganglion neurons coexpress alpha9 and alpha10 nicotinic receptor subunits, thereby providing the first example of neuronal expression of this receptor subunit pair.
先前的结合研究表明，背根神经节中存在一种迄今未知的烟碱型乙酰胆碱受体亚基（Pugh等人，1995年）。在此，我们研究了最近鉴定出的亚基α10及其潜在的相互作用伙伴α9（Elgoyhen等人，2001年）是否在这些神经节中表达。在原位杂交中，大鼠背根神经节的所有神经元而非神经胶质细胞均表达α9和α10 mRNA，并使用新制备的抗体显示出α10免疫反应性。这些发现通过逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法得到了证实。数据表明，背根神经节神经元共同表达α9和α10烟碱型受体亚基，从而提供了该受体亚基对在神经元中表达的首个实例。
