Lips Katrin Susanne, König Peter, Schätzle Katrin, Pfeil Uwe, Krasteva Gabriela, Spies Markus, Haberberger Rainer Viktor, Grando Sergei A, Kummer Wolfgang
Institute for Anatomy and Cell Biology and University of Giessen Lung Center, Justus-Liebig-University, 35385 Giessen, Germany.
J Mol Neurosci. 2006;30(1-2):15-6. doi: 10.1385/JMN:30:1:15.
Fast excitatory synaptic transmission in sympathetic ganglia is mediated by nicotinic acetylcholine receptors (nAChRs). Although it is known that the nAChR alpha7-subunit occurs in sympathetic ganglia, the expression of the recently cloned subunit alpha10 (Elgoyhen et al., 2001; Lustig et al., 2001; Sgard et al., 2002) has not been analyzed. Until now, functional receptors containing alpha10-subunits have been found only in combination with alpha9-subunits (Elgoyhen et al., 2001; Lustig et al., 2001; Sgard et al., 2002). The alpha9-subunit exhibits a restricted expression pattern, whereas the alpha10-subunit is expressed more widely. This broad distribution resembles more closely that known for subunit alpha7 than for subunit alpha9. On this background, we investigated the distribution of nAChR subunits alpha7, alpha9, and alpha10 in rat sympathetic ganglia and studied a possible interaction between subunit alpha7 and potential partners by double-labeling immunofluorescence and fluorescence resonance energy transfer (FRET) (Kam et al., 1995; Jares-Erijman and Jovin, 2003).
交感神经节中的快速兴奋性突触传递由烟碱型乙酰胆碱受体（nAChRs）介导。虽然已知nAChRα7亚基存在于交感神经节中，但最近克隆的α10亚基（Elgoyhen等人，2001年；Lustig等人，2001年；Sgard等人，2002年）的表达尚未得到分析。到目前为止，含有α10亚基的功能性受体仅在与α9亚基结合时被发现（Elgoyhen等人，2001年；Lustig等人，2001年；Sgard等人，2002年）。α9亚基表现出有限的表达模式，而α10亚基的表达更为广泛。这种广泛的分布与α7亚基的分布更为相似，而与α9亚基的分布不同。在此背景下，我们研究了nAChRα7、α9和α10亚基在大鼠交感神经节中的分布，并通过双标免疫荧光和荧光共振能量转移（FRET）（Kam等人，1995年；Jares-Erijman和Jovin，2003年）研究了α7亚基与潜在伙伴之间可能的相互作用。
