Sanders Sara R, Cuneo S Peder, Turzillo Adele M
Department of Physiology, University of Arizona, PO Box 245051, 85724-4347, Tucson, AZ, USA
Reprod Toxicol. 2002 Nov-Dec;16(6):795-800. doi: 10.1016/s0890-6238(02)00049-7.
The purpose of this study was to determine if nicotine or cotinine inhibits steroidogenesis in the ovarian follicle. Theca interna and granulosa cells were isolated from bovine follicles, cultured with nicotine or cotinine for 24h, and culture media were assayed for androstenedione or estradiol. Treatment of theca interna with 6, 60, and 600 micro M nicotine decreased (P<or=0.002) production of androstenedione to 55, 53, and 24% of control levels, respectively. Levels of androstenedione in theca interna treated with cotinine were not different from control values. In granulosa cells, nicotine inhibited production of estradiol at the highest dose tested. Treatment with 600 micro M nicotine decreased (P<or=0.001) estradiol concentration to 12% of control values, attributable to a general cytotoxic effect. Cotinine had no effect on estradiol production by granulosa cells. These results provide novel evidence for inhibitory effects of nicotine on androgen production by theca interna.
本研究的目的是确定尼古丁或可替宁是否会抑制卵巢卵泡中的类固醇生成。从牛卵泡中分离出卵泡内膜细胞和颗粒细胞，用尼古丁或可替宁培养24小时，然后检测培养基中的雄烯二酮或雌二醇。用6、60和600微摩尔/升尼古丁处理卵泡内膜细胞，雄烯二酮的产量分别降至对照水平的55%、53%和24%（P≤0.002）。用可替宁处理的卵泡内膜细胞中雄烯二酮水平与对照值无差异。在颗粒细胞中，尼古丁在测试的最高剂量下抑制雌二醇的产生。用600微摩尔/升尼古丁处理使雌二醇浓度降至对照值的12%（P≤0.001），这归因于一般的细胞毒性作用。可替宁对颗粒细胞产生雌二醇没有影响。这些结果为尼古丁对卵泡内膜细胞雄激素生成的抑制作用提供了新的证据。
