Siti Norashikin M T, Ghosh S, Chatterjee R, Rajikin M H, Chatterjee A
Faculty of Medicine Universiti Teknologi MARA (UiTM), Sungai Buloh Campus, Jalan Hospital 47000 Sungai Buloh Selangor Malaysia.
Reprod Med Biol. 2014 Jan 4;13(3):135-141. doi: 10.1007/s12522-013-0174-9. eCollection 2014 Jul.
The present study aims to investigate the effects of nicotine on the endometrial decidual growth and levels of estrogen and progesterone in pseudopregnant rats.
Pseudopregnancy (pc) was induced in cyclic Sprague-Dawley rats by sterile mating. Subcutaneous injection of nicotine tartrate (7.5 mg/kg/day) was scheduled from day 1 through day 5, day 5 through day 9 or day 1 through day 9 of pc. In another group of pseudopregnant rats, concomitant treatment of nicotine tartrate concurrently with progesterone (2 mg/day) was scheduled from day 1 through day 9 pc. Control groups received subcutaneous injections of vehicle only. Endometrial decidualization was induced on day 5 pc. On day 10 pc, animals were sacrificed.The degree of decidual growth and circulating levels of estrogen and progesterone were measured.
The decidual growth in all the first three nicotine-treated groups of animals was significantly reduced, particularly in the animals treated with nicotine from day 1 through day 9 pc. Plasma estrogen levels were significantly elevated in animals treated with nicotine from day 1 through day 9 pc. Conversely, levels of plasma progesterone were found to be significantly attenuated in the same group of nicotine-treated animals compared to controls. Exogenous replacement of progesterone, however, caused a higher degree of endometrial decidualization compared to the nicotine-treated group but it was slightly less than when compared to control.
In conclusion, nicotine-induced progesterone deficiency with a corresponding elevation of estrogen may possibly attenuate the degree of endometrial decidualization in pseudopregnant rats.
本研究旨在探讨尼古丁对假孕大鼠子宫内膜蜕膜生长及雌激素和孕酮水平的影响。
通过无菌交配诱导周期性的斯普拉格-道利大鼠假孕。从假孕第1天至第5天、第5天至第9天或第1天至第9天皮下注射酒石酸尼古丁（7.5毫克/千克/天）。在另一组假孕大鼠中，从假孕第1天至第9天安排酒石酸尼古丁与孕酮（2毫克/天）同时给药。对照组仅接受皮下注射赋形剂。在假孕第5天诱导子宫内膜蜕膜化。在假孕第10天处死动物。测量蜕膜生长程度以及雌激素和孕酮的循环水平。
前三个尼古丁处理组动物的蜕膜生长均显著降低，尤其是在从假孕第1天至第9天接受尼古丁处理的动物中。从假孕第1天至第9天接受尼古丁处理的动物血浆雌激素水平显著升高。相反，与对照组相比，同一组尼古丁处理动物的血浆孕酮水平显著降低。然而，与尼古丁处理组相比，外源性补充孕酮导致子宫内膜蜕膜化程度更高，但略低于对照组。
总之，尼古丁诱导的孕酮缺乏伴雌激素相应升高可能会减弱假孕大鼠子宫内膜蜕膜化程度。
