Wernicke Catrin, Smolka Michael, Gallinat Jürgen, Winterer Georg, Schmidt Lutz G, Rommelspacher Hans
Department of Clinical Neurobiology, Benjamin Franklin Medical School, Free University of Berlin, Ulmenallee 32, D-14050 Berlin, Germany.
Neurosci Lett. 2002 Nov 15;333(1):45-8. doi: 10.1016/s0304-3940(02)00985-0.
Two new polymorphisms in the 3' untranslated region (3'UTR) of the dopamine transporter (DAT1) gene, adjacent to the known variable number of tandem repeats (VNTR) polymorphism, have been investigated in the present population-based association study including 351 alcoholics and 336 controls. The DraI restriction site was not polymorphic in our population. The G2319A polymorphism was not significantly different with respect to genotype or allele distribution between alcoholics and controls. Subsequently, in individuals with VNTR homozygosity for the ten repeat allele, we found a higher prevalence of A/A homozygosity in patients with seizure history (P = 0.001, odds ratio (OR) = 7.913), with delirium history (P = 0.032, OR = 4.707), and with an alcoholic mother (P = 0.021, OR = 5.250), compared to homozygote 10/10 controls. Our findings provide further evidence that the 3'UTR of the DAT1 gene affects vulnerability to severe alcohol withdrawal.
在这项基于人群的关联研究中，对多巴胺转运体（DAT1）基因3'非翻译区（3'UTR）中与已知可变串联重复序列（VNTR）多态性相邻的两个新多态性进行了研究，该研究纳入了351名酗酒者和336名对照。DraI限制性酶切位点在我们的人群中没有多态性。G2319A多态性在酗酒者和对照之间的基因型或等位基因分布上没有显著差异。随后，在具有十个重复等位基因的VNTR纯合子个体中，我们发现有癫痫病史的患者（P = 0.001，优势比（OR）= 7.913）、有谵妄病史的患者（P = 0.032，OR = 4.707）以及母亲为酗酒者的患者（P = 0.021，OR = 5.250）中，A/A纯合子的患病率高于10/10纯合子对照。我们的研究结果进一步证明，DAT1基因的3'UTR会影响严重酒精戒断的易感性。
