Uhríková Daniela, Rapp Gert, Balgavý Pavol
Department of Physical Chemistry of Drugs, Faculty of Pharmacy, J A Comenius University, Odbojárov 10, SK-832 32, Bratislava, Slovak Republic.
Bioelectrochemistry. 2002 Nov;58(1):87-95. doi: 10.1016/s1567-5394(02)00122-6.
We report on a small-angle synchrotron X-ray diffraction study of dilauroylphosphatidylcholine (DLPC) liposomes aggregated with high molecular DNA in the presence of 1,4-butanediammonium-N,N'-dilauryl-N,N,N',N'-tetramethyl gemini surfactant cations (C12GS). The aggregates prepared at the DLPC/C12GS/DNA phosphate group=2:1:1.6 molar ratio in 0.0015 mol x l(-1) NaCl aqueous solution exhibit Bragg reflections due to lamellar lipid bilayer stacking and the Bragg reflection typical of one-dimensional DNA lattice with parallel strands intercalated between lipid bilayers. In this condensed fluid lamellar L(alpha)(c) phase, the interactions between DNA and charged bilayers damp the thermally induced bilayer undulations. The diffraction data obtained with the mixture of DLPC liposomes and DNA (at DNA phosphate group/DLPC=0.8:1 molar ratio) indicate a DNA-lipid interaction in the absence of C12GS.
我们报道了在1,4 - 丁二铵 - N,N'- 二月桂酰 - N,N,N',N'- 四甲基双子表面活性剂阳离子（C12GS）存在下，与高分子量DNA聚集的二月桂酰磷脂酰胆碱（DLPC）脂质体的小角同步加速器X射线衍射研究。在0.0015 mol·L⁻¹ NaCl水溶液中，以DLPC/C12GS/DNA磷酸基团 = 2:1:1.6的摩尔比制备的聚集体，由于层状脂质双层堆积而呈现布拉格反射，以及具有平行链插入脂质双层之间的一维DNA晶格典型的布拉格反射。在这种凝聚流体层状L(α)(c)相中，DNA与带电双层之间的相互作用抑制了热诱导的双层波动。用DLPC脂质体和DNA的混合物（DNA磷酸基团/DLPC = 0.8:1摩尔比）获得的衍射数据表明在不存在C12GS的情况下存在DNA - 脂质相互作用。
