Nagatsuka Hitoshi, Siar Chong Huat, Nakano Keisuke, Tsujigiwa Hidetsugu, Gunduz Mehmet, Choufuku Hoh, Lee You-Jin, Naito Ichiro, Sado Yoshikazu, Nagai Noriyuki
Department of Oral Pathology and Medicine, Graduate School of Medicine and Dentistry, Okayama University, 2-5-1 Shikata-cho, Okayama 700-8525, Japan.
Virchows Arch. 2002 Oct;441(4):392-9. doi: 10.1007/s00428-002-0658-1. Epub 2002 Jul 6.
Type IV collagen, the major component of basement membrane (BM), demonstrates a stage- and position-specific distribution of its isoforms during tooth development. To determine its localization in BM of odontogenic neoplasms, immunohistochemistry using six anti-alpha(IV) chain-specific monoclonal antibodies was performed. Results disclosed that BM demonstrated an irregular alpha(IV) chain profile in malignant odontogenic tumors as compared to benign odontogenic neoplasms. No alpha3(IV) chains were detected. Expression of alpha1(IV)/alpha2(IV) and alpha5(IV)/alpha6(IV) chains was stronger in desmoplastic ( n=3) than in ordinary (n=5) ameloblastomas. The adenomatoid odontogenic tumor ( n=2) distinctly expressed these chains in BM of cribriform areas and hyaline materials (which was also alpha4(IV)-positive), but weakly around epithelial whorls/rosettes/nests and mineralized foci. These five chains also stained BM and tumor cells of ameloblastic fibroma ( n=3) and ameloblastic fibro-odontosarcoma (n=1), but not the inductive hard tissues. Ameloblastic carcinoma ( n=2) showed specific alpha1(IV)/alpha2(IV) chain loss, while primary intraosseous carcinoma ( n=1) demonstrated a discontinuous alpha1(IV)/alpha2(IV) and alpha5(IV)/alpha6(IV) staining pattern. The present results suggest that modification and remodeling of BM collagen IValpha chains occur during odontogenic neoplasms' progression.
IV型胶原是基底膜（BM）的主要成分，在牙齿发育过程中，其异构体呈现出阶段和位置特异性分布。为了确定其在牙源性肿瘤基底膜中的定位，使用六种抗α(IV)链特异性单克隆抗体进行了免疫组织化学检测。结果显示，与良性牙源性肿瘤相比，恶性牙源性肿瘤的基底膜呈现出不规则的α(IV)链分布模式。未检测到α3(IV)链。在促结缔组织增生型成釉细胞瘤（n = 3）中，α1(IV)/α2(IV)和α5(IV)/α6(IV)链的表达强于普通型成釉细胞瘤（n = 5）。腺样牙源性肿瘤（n = 2）在筛状区域和透明物质的基底膜中明显表达这些链（该透明物质也呈α4(IV)阳性），但在上皮性漩涡/玫瑰花结/巢以及矿化灶周围表达较弱。这五条链也对成釉细胞纤维瘤（n = 3）和成釉细胞纤维-牙肉瘤（n = 1）的基底膜和肿瘤细胞进行了染色，但未对诱导性硬组织染色。成釉细胞癌（n = 2）显示出特异性的α1(IV)/α2(IV)链缺失，而原发性骨内癌（n = 1）表现出α1(IV)/α2(IV)和α5(IV)/α6(IV)染色模式的不连续。目前的结果表明，在牙源性肿瘤进展过程中发生了基底膜胶原IVα链的修饰和重塑。
