Pagan J D, Geor R J, Harris P A, Hoekstra K, Gardner S, Hudson C, Prince A
Kentucky Equine Research, Versailles 40383, USA.
Equine Vet J Suppl. 2002 Sep(34):33-8. doi: 10.1111/j.2042-3306.2002.tb05388.x.
This study was designed to determine the effects of fat adaptation on carbohydrate and fat oxidation in conditioned horses during low-intensity exercise. Five mature Arabians were studied. The study was conducted as a crossover design with 2 dietary periods, each of 10 week's duration: a) a control (CON) diet, and b) a fat-supplemented (FAT) diet. The total amount of digestible energy (DE) supplied by the fat in the CON and FAT diets was 7% and 29%, respectively. During each period, the horses completed exercise tests at the beginning of the period (Week 0) and after 5 and 10 weeks on the diet. Tests consisted of 90 min of exercise at a speed calculated to elicit 35% VO2max on a treadmill inclined to 3 degrees. Oxygen consumption (VO2), carbon dioxide production (VCO2), and respiratory exchange ratio (RER) were measured at 15-min intervals. For determination of glucose kinetics, a stable isotope ([6-6-d2] glucose) technique was used. Compared to the CON diet, FAT diet consumption for 5-10 weeks was associated with an altered metabolic response to low-intensity exercise, as evidenced by a more than 30% reduction in the production and utilisation of glucose; a decrease in RER; a decrease in the estimated rate of whole-body carbohydrate utilisation; and an increase in the whole-body rate of lipid oxidation during exercise.
本研究旨在确定脂肪适应对训练有素的马匹在低强度运动期间碳水化合物和脂肪氧化的影响。研究了5匹成年阿拉伯马。本研究采用交叉设计，有2个饮食阶段，每个阶段持续10周：a）对照（CON）饮食，和b）补充脂肪（FAT）饮食。CON饮食和FAT饮食中脂肪提供的可消化能量（DE）总量分别为7%和29%。在每个阶段，马匹在阶段开始时（第0周）以及饮食5周和10周后完成运动测试。测试包括在倾斜3度的跑步机上以计算得出的能引发35%最大摄氧量（VO2max）的速度进行90分钟的运动。每隔15分钟测量一次耗氧量（VO2）、二氧化碳产生量（VCO2）和呼吸交换率（RER）。为了测定葡萄糖动力学，使用了稳定同位素（[6-6-d2]葡萄糖）技术。与CON饮食相比，5至10周的FAT饮食消耗与对低强度运动的代谢反应改变有关，表现为葡萄糖的产生和利用减少30%以上；RER降低；全身碳水化合物利用估计速率降低；以及运动期间全身脂质氧化速率增加。
