Grobuschek Nina, Sriphong Lawan, Schmid Martin G, Lorànd Tamàs, Aboul-Enein Hassan Y, Gübitz Gerald
Institute of Pharmaceutical Chemistry and Pharmaceutical Technology, Karl-Franzens University, Universitätsplatz 1, A-8010 Graz, Austria.
J Biochem Biophys Methods. 2002 Oct-Nov;53(1-3):25-36. doi: 10.1016/s0165-022x(02)00089-1.
The chiral separation of cyclic Mannich ketones of potential pharmaceutical interest is investigated using HPLC and CE. These Mannich ketones show a marked antibacterial and antifungal activity. In HPLC, stationary phases containing cellulose derivatives or beta-cyclodextrin were used and in CE different cyclodextrins, such as beta-CD, gamma-CD, carboxymethyl-beta-CD and succinyl-beta-CD were added to the background electrolyte as chiral selectors.
采用高效液相色谱法（HPLC）和毛细管电泳法（CE）研究了具有潜在药学意义的环状曼尼希酮的手性拆分。这些曼尼希酮表现出显著的抗菌和抗真菌活性。在HPLC中，使用了含有纤维素衍生物或β-环糊精的固定相，而在CE中，将不同的环糊精，如β-环糊精、γ-环糊精、羧甲基-β-环糊精和琥珀酰-β-环糊精作为手性选择剂添加到背景电解质中。
