镍缺乏对产甲烷颗粒污泥生物反应器中甲醇降解的影响。

Effect of nickel deprivation on methanol degradation in a methanogenic granular sludge bioreactor.

作者信息

Zandvoort M H, Osuna M B, Geerts R, Lettinga G, Lens P N L

机构信息

Subdepartment of Environmental Technology, Agricultural University of Wageningen, "Biotechnion"- Bomenweg, 2, PO Box 8129, Wageningen 6700 EV, The Netherlands.

出版信息

J Ind Microbiol Biotechnol. 2002 Nov;29(5):268-74. doi: 10.1038/sj.jim.7000311.

DOI:10.1038/sj.jim.7000311

PMID:12407462

Abstract

The effect of omitting nickel from the influent on methanol conversion in an Upflow Anaerobic Sludge Bed (UASB) reactor was investigated. The UASB reactor (30 degrees C, pH 7) was operated for 261 days at a 12-h hydraulic retention time (HRT) and at organic loading rates (OLRs) ranging from 2.6 to 7.8 g COD l reactor(-1) day(-1). The nickel content of the sludge decreased by 66% during the 261-day reactor run because of washout and doubling of the sludge bed volume. Nickel deprivation initially had a strong impact on the methanogenic activity of the sludge with methanol; e.g., after 89 days of operation, this activity was doubled by adding 2 micro M nickel. Upon prolonged UASB reactor operation, methanol and VFA effluent concentrations decreased whereas the sludge lost its response to nickel addition in activity tests. This suggests that a less nickel-dependent methanol-converting sludge had developed in the UASB reactor.

摘要

研究了进水不含镍对升流式厌氧污泥床（UASB）反应器中甲醇转化的影响。UASB反应器（30℃，pH 7）在水力停留时间（HRT）为12小时、有机负荷率（OLR）为2.6至7.8 g COD l反应器⁻¹天⁻¹的条件下运行了261天。由于污泥流失和污泥床体积加倍，在261天的反应器运行期间，污泥中的镍含量下降了66%。缺镍最初对污泥利用甲醇的产甲烷活性有强烈影响；例如，运行89天后，通过添加2 μM镍，该活性提高了一倍。随着UASB反应器的长期运行，甲醇和挥发性脂肪酸（VFA）的出水浓度降低，而在活性测试中污泥对添加镍的反应消失。这表明UASB反应器中已形成了对镍依赖性较低的甲醇转化污泥。