Kitahashi Toshihiro, Furuta Itaru
Department of Laboratory Medicine, Kinki University School of Medicine, 377-2 Ohno-higashi, Osakasayama, Osaka, Japan.
J Pharm Biomed Anal. 2002 Nov 7;30(4):1411-6. doi: 10.1016/s0731-7085(02)00475-2.
A precise method for determining linezolid concentration in human serum by micellar electrokinetic capillary chromatography has been developed and validated. Serum was deproteinized with acetonitrile and etofylline was used as an internal standard. A borate buffer (pH 10.0; 25 mM) containing sodium dodecyl sulfate (80 mM) was used as a running buffer. Detection was performed at UV253 nm by applying 25 kV voltage to a fused-silica capillary tube. Migration time of linezolid was approximately 14 min. Good linearity (0-100 mg/l) was obtained and the limit of detection was 0.5 mg/l (S/N=3). This technique covered the clinical concentration (4 mg/l) measurement of this drug enough. The intra- and inter-day reproducibility was good. Serum recovery was 95-102%. No interference from other anti-microbial agents was observed. Linezolid after serum deproteinization showed high stability. This method was easy to operate as well as economical as a method for determining linezolid in serum.
已开发并验证了一种通过胶束电动毛细管色谱法测定人血清中利奈唑胺浓度的精确方法。血清用乙腈进行脱蛋白处理，并使用依托菲林作为内标。含有十二烷基硫酸钠（80 mM）的硼酸盐缓冲液（pH 10.0；25 mM）用作运行缓冲液。通过向熔融石英毛细管施加25 kV电压在UV253 nm处进行检测。利奈唑胺的迁移时间约为14分钟。获得了良好的线性（0 - 100 mg/l），检测限为0.5 mg/l（S/N = 3）。该技术足以涵盖该药物的临床浓度（4 mg/l）测量。日内和日间重现性良好。血清回收率为95 - 102%。未观察到其他抗菌剂的干扰。血清脱蛋白后的利奈唑胺显示出高稳定性。作为一种测定血清中利奈唑胺的方法，该方法操作简便且经济。
