Kitahashi Toshihiro, Furuta Itaru
Department of Laboratory Medicine, Kinki University School of Medicine, 377-2 Ohno-higashi, Osakasayama, Osaka, Japan.
J Pharm Biomed Anal. 2004 Jan 27;34(1):153-8. doi: 10.1016/j.japna.2003.08.019.
Iohexol is widely used in clinical laboratories as a non-ionic radiographic contrast medium. Determination of its concentration in blood has a vital meaning in preventing its side effects caused by its retention in the system. A method for determining iohexol in serum by micellar electrokinetic capillary chromatography (MECC) requiring no pretreatment is developed. Electrophoresis is performed for serum samples at 25 kV with a borate buffer (50mM; pH 9.5) containing sodium dodecyl sulfate (50mM) and detection is carried out at 245 nm. Migration time of iohexol is 7.4 min. Linearity (0-1000 mg/l) is good and detection limit is 0.5mg/l (S/N=3). CV of intra-assay precision at a measurement concentration range of 6.2-200.1mg/l is 1.38-4.68% and recovery rate is 96-102%. CV of inter-assay precision is 2.06-5.94% at a measurement concentration range of 10.3-155.4 mg/l. This method is characterized by determination through direct injection of serum samples of super micro-quantity into the capillary, which simplifies the determination procedure in a significant manner and improves the precision and accuracy of determination.
碘海醇作为一种非离子型造影剂在临床实验室中被广泛使用。测定其在血液中的浓度对于预防因体内潴留而引起的副作用具有至关重要的意义。本文建立了一种无需预处理即可通过胶束电动毛细管色谱法（MECC）测定血清中碘海醇的方法。血清样品在含十二烷基硫酸钠（50mM）的硼酸盐缓冲液（50mM；pH 9.5）中于25 kV电压下进行电泳，并在245 nm波长处进行检测。碘海醇的迁移时间为7.4分钟。线性范围（0 - 1000 mg/l）良好，检测限为0.5mg/l（S/N = 3）。在6.2 - 200.1mg/l的测量浓度范围内，批内精密度的变异系数（CV）为1.38 - 4.68%，回收率为96 - 102%。在10.3 - 155.4 mg/l的测量浓度范围内，批间精密度的CV为2.06 - 5.94%。该方法的特点是将超微量血清样品直接进样到毛细管中进行测定，显著简化了测定程序，提高了测定的精密度和准确性。
