Cai Yu, Jia Jun-Wei, Crock John, Lin Zhi-Xin, Chen Xiao-Ya, Croteau Rodney
Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, P R, China.
Phytochemistry. 2002 Nov;61(5):523-9. doi: 10.1016/s0031-9422(02)00265-0.
An homology-based cloning strategy yielded a full-length cDNA from Artemisia annua that encoded a protein of 60.3 kDa which resembled a sesquiterpene synthase in sequence. Heterologous expression of the gene in Escherichia coli provided a soluble recombinant enzyme capable of catalyzing the divalent metal ion-dependent conversion of farnesyl diphosphate to beta-caryophyllene, a sesquiterpene olefin found in the essential oil of A. annua. In reaction parameters and kinetic properties, beta-caryophyllene synthase resembles other sesquiterpene synthases of angiosperms. The beta-caryophyllene synthase gene is expressed in most plant tissues during early development, and is induced in mature tissue in response to fungal elicitor thus suggesting a role for beta-caryophyllene in plant defense.
一种基于同源性的克隆策略从黄花蒿中获得了一个全长cDNA，该cDNA编码一种60.3 kDa的蛋白质，其序列类似于倍半萜合酶。该基因在大肠杆菌中的异源表达产生了一种可溶性重组酶，该酶能够催化法呢基二磷酸向β-石竹烯的二价金属离子依赖性转化，β-石竹烯是黄花蒿精油中发现的一种倍半萜烯烃。在反应参数和动力学特性方面，β-石竹烯合酶类似于被子植物的其他倍半萜合酶。β-石竹烯合酶基因在早期发育过程中在大多数植物组织中表达，并在成熟组织中响应真菌激发子而被诱导，因此表明β-石竹烯在植物防御中发挥作用。
