Balasubramani Sundararajan, Ranjitha Kumari B D, Moola Anil Kumar, Sathish D, Prem Kumar G, Srimurali S, Babu Rajendran R
College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.
Department of Botany, Bharathidasan University, Tiruchirappalli, India.
Front Plant Sci. 2021 Mar 30;12:634178. doi: 10.3389/fpls.2021.634178. eCollection 2021.
L. produces a wide range of valuable secondary metabolites. The aim of the present study is to determine the effects of various concentrations of farnesyl diphosphate (FDP) on β-caryophyllene content in both callus and hairy root (HR) cultures regeneration from leaf explants of L. Murashige and Skoog (MS) medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2,4D; 4-13 μM), α-naphthaleneacetic acid (NAA; 5-16 μM), and FDP (1 and 3 μM) was used for callus induction and HR regeneration from leaf explants of L. In this study, precursor-treated (2,4D 13.5 μM + FDP 3 μM) callus displayed the highest biomass fresh weight (FW)/dry weight (DW): 46/25 g, followed by NAA 10.7 μM + FDP 3 μM with FW/DW: 50/28 g. Two different strains (A and R) were evaluated for HR induction. The biomass of HRs induced using half-strength MS + B vitamins with 3 μM FDP was FW/DW: 40/20 g and FW/DW: 41/19 g, respectively. To determine β-caryophyllene accumulation, we have isolated the essential oil from FDP-treated calli and HRs and quantified β-caryophyllene using gas chromatography-mass spectrometry (GC-MS). The highest production of β-caryophyllene was noticed in HR cultures induced using A and R strains on half-strength MS medium containing 3 μM FDP, which produced 2.92 and 2.80 mg/ml β-caryophyllene, respectively. The optimized protocol can be used commercially by scaling up the production of a β-caryophyllene compound in a short span of time.
罗勒（Ocimum basilicum L.）能产生多种有价值的次生代谢产物。本研究的目的是确定不同浓度的法尼基二磷酸（FDP）对罗勒叶外植体愈伤组织和毛状根（HR）培养物中β-石竹烯含量的影响。在添加不同浓度2,4-二氯苯氧乙酸（2,4-D；4 - 13 μM）、α-萘乙酸（NAA；5 - 16 μM）和FDP（1和3 μM）的Murashige和Skoog（MS）培养基上，从罗勒叶外植体诱导愈伤组织并进行HR再生。在本研究中，经前体处理（2,4-D 13.5 μM + FDP 3 μM）的愈伤组织显示出最高的生物量鲜重（FW）/干重（DW）：46/25 g，其次是NAA 10.7 μM + FDP 3 μM，FW/DW为50/28 g。评估了两种不同菌株（A和R）用于HR诱导的情况。使用含3 μM FDP的半强度MS + B族维生素诱导的HR生物量，FW/DW分别为40/20 g和41/19 g。为了确定β-石竹烯的积累情况，我们从经FDP处理的愈伤组织和HR中分离出挥发油，并使用气相色谱 - 质谱联用仪（GC-MS）对β-石竹烯进行定量。在含有3 μM FDP的半强度MS培养基上，使用A和R菌株诱导的HR培养物中，β-石竹烯的产量最高，分别产生2.92和2.80 mg/ml的β-石竹烯。通过在短时间内扩大β-石竹烯化合物的生产规模，优化后的方案可用于商业生产。
