Németh József, Jakab Balázs, Reglodi Dóra, Lubics Andrea, Józsa Rita, Hollósy Tibor, Tamás Andrea, Lengvári István, Görcs Tamás, Szolcsányi János
Department of Pharmacology and Pharmacotherapy, Neuropharmacology Research Group of the Hungarian Academy of Sciences, University of Pécs, P.O. Box 99, H-7643, Pécs, Hungary.
Regul Pept. 2002 Nov 15;109(1-3):3-7. doi: 10.1016/s0167-0115(02)00165-9.
Vasoactive intestinal polypeptide (VIP) occurs in high concentrations throughout the gut and the nervous system. The presence of VIP has been shown in a number of species, mainly by immunohistochemistry. The aim of the present study was to develop a new, highly specific VIP radioimmunoassay to investigate the distribution of VIP in the central nervous system of various vertebrate and invertebrate species. Different areas of the brain and spinal cord were removed from rats, chickens, turtles, frogs and fishes. The cerebral ganglia and the ventral ganglionic chain were investigated in the earthworm. The tissue samples were processed for VIP radioimmunoassay. Our results show that the antiserum used in the radioimmunoassay turned to be C-terminal specific, without significant affinity to other members of the VIP peptide family. Detection limit of the assay was 0.1 fmol/ml. Highest concentrations were found in the turtle diencephalon, followed by other brain areas in the turtle and rat. All other brain areas in the examined species contained significant levels of VIP. Immunoreactivity was also shown in the cerebral and ventral ganglia of the earthworm. In summary, our results show comparative quantitative distribution in representative species of the phylogenetic line, using the same experimental conditions.
血管活性肠肽（VIP）在整个肠道和神经系统中都以高浓度存在。VIP的存在已在许多物种中得到证实，主要是通过免疫组织化学方法。本研究的目的是开发一种新的、高度特异性的VIP放射免疫测定法，以研究VIP在各种脊椎动物和无脊椎动物物种中枢神经系统中的分布。从大鼠、鸡、龟、蛙和鱼身上取出大脑和脊髓的不同区域。对蚯蚓的脑神经节和腹神经节链进行了研究。对组织样本进行处理以进行VIP放射免疫测定。我们的结果表明，放射免疫测定中使用的抗血清是C末端特异性的，对VIP肽家族的其他成员没有明显亲和力。该测定法的检测限为0.1飞摩尔/毫升。在龟的间脑中发现浓度最高，其次是龟和大鼠的其他脑区。在所检查物种的所有其他脑区中都含有显著水平的VIP。在蚯蚓的脑神经节和腹神经节中也显示出免疫反应性。总之，我们的结果表明，在相同的实验条件下，在系统发育线的代表性物种中存在比较性的定量分布。
